Molecular basis of resistance to leaf spot disease in oil palm.

IF 4.1 2区生物学 Q1 PLANT SCIENCES

Frontiers in Plant Science Pub Date : 2024-12-09 eCollection Date: 2024-01-01 DOI:10.3389/fpls.2024.1458346

Cahyo S Wibowo, Ricki Susilo, Reza Ernawan, Ardha Apriyanto, Mohammed O Alshaharni, Graham R Smith, Angharad M R Gatehouse, Martin G Edwards

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引用次数: 0

Abstract

Introduction: Leaf spot disease caused by the fungal pathogen Curvularia oryzae is one of the most common diseases found in oil palm (Elaeis guineensis) nurseries in South East Asia, and is most prevalent at the seedling stage. Severe infections result in localized necrotic regions of leaves that rapidly spread within nurseries leading to poor quality seedlings and high economic losses.

Methods: To understand the molecular mechanisms of this plant-pathogen interaction, RNA-Seq was used to elucidate the transcriptomes of three oil palm genotypes with contrasting pathogen responses (G10 and G12, resistant and G14, susceptible) following infection with C. oryzae spores. Transcriptomes were obtained from Illumina NovaSeq 6000 sequencing of mRNA at four different time points (day 0, before treatment; day 1, 7, and 21 post treatment).

Results and discussion: Analysis of differentially expressed gene (DEG) profiles in these three genotypes provided an overview of the genes involved in the plant defence. Genes involved in disease resistance, phytohormone biosynthesis, gene regulation (transcription factors), and those encoding proteins associated with cell wall hardening were identified and likely contribute to the resistance of oil palm to C. oryzae. Such genes represent good candidates for targets to enhance oil palm productivity and resilience through molecular breeding approaches.

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来源期刊

Frontiers in Plant Science PLANT SCIENCES-

CiteScore

7.30

自引率

14.30%

发文量

4844

审稿时长

14 weeks

期刊介绍： In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches. Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.