Evaluation of stripe rust resistance and analysis of resistance genes in wheat genotypes from Pakistan and Southwest China.

IF 4.1 2区生物学 Q1 PLANT SCIENCES

Frontiers in Plant Science Pub Date : 2024-12-09 eCollection Date: 2024-01-01 DOI:10.3389/fpls.2024.1494566

Sakina Abbas, Yunfang Li, Jing Lu, Jianming Hu, Xinnuo Zhang, Xue Lv, Armghan Shahzad, Donghui Ao, Maryam Abbas, Yu Wu, Lei Zhang, Muhammad Fayyaz

{"title":"Evaluation of stripe rust resistance and analysis of resistance genes in wheat genotypes from Pakistan and Southwest China.","authors":"Sakina Abbas, Yunfang Li, Jing Lu, Jianming Hu, Xinnuo Zhang, Xue Lv, Armghan Shahzad, Donghui Ao, Maryam Abbas, Yu Wu, Lei Zhang, Muhammad Fayyaz","doi":"10.3389/fpls.2024.1494566","DOIUrl":null,"url":null,"abstract":"Introduction: Stripe rust, caused by Puccinia striiformis f. sp. tritici, poses a significant threat to wheat quality and production worldwide. The rapid evolution of Pst races caused several resistance genes to be ineffective.Methods: This study evaluated stripe rust resistance genes in 349 Pakistan and Southwest China genotypes. We utilized previously published functional and linked molecular markers to detect 13 major stripe rust resistance genes: Yr5, Yr9, Yr10, Yr15, Yr17, Yr18, Yr26, Yr29, Yr30, Yr36, Yr48, Yr65, and YrSp. Field evaluations assessed IT and resistance levels, while the impact of gene combinations on resistance was also analyzed.Results: Field evaluations showed that over 60% of Chuanyu wheat, 50% of recent Pakistani cultivars, and 20% of historic Pakistani lines were resistant to current stripe rust races. In Chuanyu wheat, the dominant genes were Yr17, YrSp, and Yr48; however, Yr17, Yr26, and YrSp were overused, while Yr36 was absent, and Yr18 was rare. In historic lines, Yr5, Yr17, Yr18, and Yr26 were prevalent, with Yr15, Yr26, and YrSp demonstrating effective resistance against current stripe rust races. Furthermore, the study identified specific combinations of Yr genes (Yr26+Yr48, Yr29+Yr5, Yr26+Yr30, and Yr30+Yr17) that enhanced resistance to Pst.Discussion: This research highlights effective resistance genes and gene combinations for stripe rust in wheat and emphasizes the deployment of durable resistance. The findings guide the strategic use of these genes in breeding programs aimed at developing durable resistance in wheat genotypes in Pakistan and Southwest China.","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"15 ","pages":"1494566"},"PeriodicalIF":4.1000,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663667/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Plant Science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fpls.2024.1494566","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: Stripe rust, caused by Puccinia striiformis f. sp. tritici, poses a significant threat to wheat quality and production worldwide. The rapid evolution of Pst races caused several resistance genes to be ineffective.

Methods: This study evaluated stripe rust resistance genes in 349 Pakistan and Southwest China genotypes. We utilized previously published functional and linked molecular markers to detect 13 major stripe rust resistance genes: Yr5, Yr9, Yr10, Yr15, Yr17, Yr18, Yr26, Yr29, Yr30, Yr36, Yr48, Yr65, and YrSp. Field evaluations assessed IT and resistance levels, while the impact of gene combinations on resistance was also analyzed.

Results: Field evaluations showed that over 60% of Chuanyu wheat, 50% of recent Pakistani cultivars, and 20% of historic Pakistani lines were resistant to current stripe rust races. In Chuanyu wheat, the dominant genes were Yr17, YrSp, and Yr48; however, Yr17, Yr26, and YrSp were overused, while Yr36 was absent, and Yr18 was rare. In historic lines, Yr5, Yr17, Yr18, and Yr26 were prevalent, with Yr15, Yr26, and YrSp demonstrating effective resistance against current stripe rust races. Furthermore, the study identified specific combinations of Yr genes (Yr26+Yr48, Yr29+Yr5, Yr26+Yr30, and Yr30+Yr17) that enhanced resistance to Pst.

Discussion: This research highlights effective resistance genes and gene combinations for stripe rust in wheat and emphasizes the deployment of durable resistance. The findings guide the strategic use of these genes in breeding programs aimed at developing durable resistance in wheat genotypes in Pakistan and Southwest China.

查看原文本刊更多论文

巴基斯坦和中国西南地区小麦基因型对条锈病的抗性评价及抗性基因分析。

小麦条锈病是由小麦条锈病引起的一种严重威胁小麦品质和产量的病害。Pst种族的快速进化导致一些抗性基因失效。方法：对巴基斯坦和中国西南地区349个基因型的抗条锈病基因进行鉴定。我们利用先前发表的功能和连锁分子标记检测了13个主要的抗条锈病基因：Yr5、Yr9、Yr10、Yr15、Yr17、Yr18、Yr26、Yr29、Yr30、Yr36、Yr48、Yr65和YrSp。实地评估评估了IT和抗性水平，同时还分析了基因组合对抗性的影响。结果：田间评价表明，60%以上的川渝小麦、50%的巴基斯坦新品种和20%的巴基斯坦历史品种对当前条锈病具有抗性。川渝小麦的优势基因为Yr17、YrSp和Yr48；但Yr17、Yr26、YrSp被过度使用，而Yr36缺失，Yr18罕见。在历史品种中，Yr5、Yr17、Yr18和Yr26普遍存在，Yr15、Yr26和YrSp对当前的条锈病品种表现出有效的抗性。此外，研究还发现了Yr基因的特定组合（Yr26+Yr48、Yr29+Yr5、Yr26+Yr30和Yr30+Yr17）增强了对Pst的抗性。讨论：本研究强调了小麦条锈病的有效抗性基因和基因组合，并强调了持久抗性的部署。研究结果指导了这些基因在巴基斯坦和中国西南地区培育持久抗性小麦基因型育种计划中的战略性使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Frontiers in Plant Science PLANT SCIENCES-

CiteScore

7.30

自引率

14.30%

发文量

4844

审稿时长

14 weeks

期刊介绍： In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches. Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.