Eco-friendly synthesis of silver nanoparticles by Trigonella foenum-graecum: formulations, characterizations, and application in wound healing.

IF 2.4 4区医学 Q3 CHEMISTRY, MEDICINAL

Drug Development and Industrial Pharmacy Pub Date : 2024-11-01 Epub Date: 2024-12-15 DOI:10.1080/03639045.2024.2431934

Amal Al-Subaiyel, Ahmed A H Abdellatif

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引用次数: 0

Abstract

Background: Due to the toxicity and serious side effects of chemical incorporated in topical dosage form used for treatment of wound healing, there is a need to use natural preparation as wound healing preparation.

Aims: Seeds of Trigonella foenum-graecum (TFG) are used to synthesize eco-friendly silver nanoparticles (SNPs) in an appropriate way to heal wounds.

Methods: To synthesize SNPs, TFG was incubated with AgNO₃ to produce SNP-TFG. The obtained SNP-TFG was characterized for their wavelength, size and ζ-potential, surface morphology, and yield production. Then, SNP-TFG was formulated as a topical cream (O/W), characterized, and applied to the rats' groups to examine its wound-healing activity. Finally, a skin biopsy was performed to assess all rats' immunostaining and histopathological (HP) alterations in skin lesions on days 3, 7, 10, and 14.

Results: The prepared SNP-TFG showed non-aggregated nano-formulation, with a λ_max of 396 nm. SNP-TFG recorded a size of 43.65 ± 2.1 nm, a charge of -15.03 ± 3.2 mV, and showed yield of 52.61 ± 1.41% while the release was continued for more than 12 h. During the biosynthesis process, the compounds present in TFG are capable of reducing silver ions (Ag⁺) to form SNPs. SNP-TFG cream showed a pH nearly equal to the skin's pH, with suitable viscosity and homogeneity and an apparent permeability of 0.009 ± 0.001. Further, the HP of the SNP-TFG showed a substantial reduction in wound mass, wound granulation tissue growth enhancement, and epidermal re-epithelialization (proliferation) compared to the control group.

Conclusion: The obtained SNP-TFG is considered a novel skin wound-healing natural and eco-friendly nano-formulation.

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利用三角三角霉环保合成纳米银颗粒：配方、表征及其在伤口愈合中的应用。

背景：由于化学制剂外用剂型用于伤口愈合治疗的毒性和严重的副作用，需要使用天然制剂作为伤口愈合制剂。目的：利用三角三角菌种子（Trigonella foenum-graecum， TFG）合成生态友好型纳米银（SNPs），用于伤口愈合。方法：用AgNO3培养TFG生成SNP-TFG合成snp。对得到的SNP-TFG进行了波长、尺寸、ζ电位、表面形貌和产率的表征。然后，将SNP-TFG配制成外用乳膏（O/W），对其进行表征，并应用于大鼠组，以检测其伤口愈合活性。最后，在第3、7、10和14天进行皮肤活检以评估所有大鼠皮肤病变的免疫染色和组织病理学（HP）改变。结果：制备的SNP-TFG呈非聚集型纳米构型，λmax为396 nm。SNP-TFG的尺寸为43.65±2.1 nm，电荷为-15.03±3.2 mV，释放时间超过12 h，产率为52.61±1.41%。在生物合成过程中，TFG中存在的化合物能够还原银离子（Ag+）形成snp。SNP-TFG乳膏的pH值与皮肤的pH值接近，具有良好的粘度和均匀性，表观渗透率为0.009±0.001。此外，与对照组相比，SNP-TFG的HP显着减少了伤口质量，伤口肉芽组织生长增强，表皮再上皮化（增殖）。结论：所得SNP-TFG是一种新型的皮肤创面愈合天然环保纳米制剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug Development and Industrial Pharmacy 医学-药学

CiteScore

6.80

自引率

0.00%

发文量

审稿时长

4.5 months

期刊介绍： The aim of Drug Development and Industrial Pharmacy is to publish novel, original, peer-reviewed research manuscripts within relevant topics and research methods related to pharmaceutical research and development, and industrial pharmacy. Research papers must be hypothesis driven and emphasize innovative breakthrough topics in pharmaceutics and drug delivery. The journal will also consider timely critical review papers.