Study on molecular response of alfalfa to low temperature stress based on transcriptomic analysis.

IF 4.3 2区 生物学 Q1 PLANT SCIENCES
Hongyu Xu, Zipei Zhang, Qingcui Zhao, Yaqi Gao, Yan Xiang, Jialong Chai, Yuying Li, Xiangyang Hou
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Abstract

Background: Alfalfa (Medicago sativa L.) is an important high-quality forage crop. Low temperature is an abiotic stress factor that affects the distribution and productivity of alfalfa. To further understand the molecular response to low temperature, and to identify additional genes and metabolic pathways associated with cold tolerance in alfalfa, in this study we conducted transcriptome sequencing, weighted gene co-expression network analysis, KEGG pathway enrichment analysis, and quantitative real-time PCR validation in alfalfa cultivars subjected to low-temperature treatment.

Results: Weighted gene co-expression network analysis revealed that three gene modules were significantly negatively correlated with the semi-lethal temperature for alfalfa. Genes in the three modules were used to construct gene co-expression networks, from which MS.gene46105, MS.gene044087, MS.gene76894, MS.gene44620, MS.gene22005, MS.gene045060, MS.gene31405, and MS.gene74761 were selected as important genes associated with cold tolerance. Quantitative real-time PCR analysis of these eight genes validated the reliability of the transcriptome sequencing data. In addition, further analysis of the genes within the three modules revealed that several transcription factors (AP2/ERF, bZIP, C3H, NAC, and others) and metabolic pathways (N-glycan biosynthesis, citrate cycle, glycolysis/gluconeogenesis, and carbon metabolism, and others) responded well to the low temperature.

Conclusions: Three gene modules, eight genes, several transcription factors and multiple metabolic pathways associated with cold tolerance were screened. This results will provide a valuable reference for further clarification of the cold tolerance mechanism and breeding for cold tolerance in alfalfa.

基于转录组学分析的紫花苜蓿低温胁迫分子响应研究。
背景:苜蓿(Medicago sativa L.)是重要的优质饲料作物。低温是影响紫花苜蓿分布和产量的非生物胁迫因子。为了进一步了解低温对苜蓿耐冷性的分子响应,确定与苜蓿耐冷性相关的其他基因和代谢途径,本研究对低温处理的苜蓿品种进行了转录组测序、加权基因共表达网络分析、KEGG通路富集分析和实时荧光定量PCR验证。结果:加权基因共表达网络分析显示,3个基因模块与苜蓿半致死温度呈显著负相关。利用3个模块中的基因构建基因共表达网络,从中筛选出MS.gene46105、MS.gene044087、MS.gene76894、MS.gene44620、MS.gene22005、MS.gene045060、MS.gene31405和MS.gene74761等与抗寒性相关的重要基因。对这8个基因进行实时定量PCR分析,验证了转录组测序数据的可靠性。此外,对三个模块内基因的进一步分析表明,一些转录因子(AP2/ERF、bZIP、C3H、NAC等)和代谢途径(n -聚糖生物合成、柠檬酸循环、糖酵解/糖异生和碳代谢等)对低温反应良好。结论:筛选到与耐寒性相关的3个基因模块、8个基因、多个转录因子和多种代谢途径。该结果将为进一步阐明紫花苜蓿的耐寒机理和选育耐寒品种提供有价值的参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Plant Biology
BMC Plant Biology 生物-植物科学
CiteScore
8.40
自引率
3.80%
发文量
539
审稿时长
3.8 months
期刊介绍: BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.
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