Identification of QTLs for early maturity-related traits based on RIL population of two elite cotton cultivars.

IF 4.3 2区 生物学 Q1 PLANT SCIENCES
Xiaoge Wang, Fanjin Kong, Liying Gao, Guifang Shen, Bing Duan, Zongwen Wang, Dongdong Xu, Degang Fan, Yongsheng Deng, Zongfu Han
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引用次数: 0

Abstract

Background: Early-maturity cotton varieties have the potential to be cultivated in a wider geographical area, extending as far north as 46 °N in China, and confer to address the issue of competition for land between grain and cotton by reducing their whole growth period (WGP). Therefore, it is of great importance to develop cotton varieties with comprehensive early maturity and high yield following investigating the regulatory mechanism underlying early maturity and identifying early maturity-related genes.

Results: In this study, 'SCRC19' and 'SCRC21', two excellent cultivars with significantly different WGP, along with their recombinant inbred lines (RILs) consisting of 150 individuals were re-sequenced, yielding 4,092,677 high-quality single nucleotide polymorphisms (SNPs) and 794 bin markers across 26 chromosomes. A genetic map spanning 2213.71 cM was constructed using the 794 bin markers. Based on this map, we identified a total of 78 early maturity-related QTLs, including 12 QTLs for WGP, 4 for SSP, 12 for SFP, 3 for FBP, 11 for NFFB, 8 for NFB, 16 for HNFFB and 12 for PH. Six QTL clusters, each containing more than four traits, were identified. One particular QTL cluster, which had the largest number of QTLs, ranged from 108.5 cM to 109 cM on Dt3, and contained 39 genes. Through functional analysis, we highlighted two early maturity-related candidates of GH_D03G1554 and GH_D03G1541, which were annotated as a BEL1-like homeodomain protein 8 and a homeobox-leucine zipper family protein, respectively.

Conclusions: We have identified a QTL cluster related to six early maturity-associated traits on Dt3. Through annotation of genes from candidate region, we have identified two candidate genes, GH_D03G1554 and GH_D03G1541, whose expression levels in 'SCRC21' were significantly higher than those in 'SCRC19' at different stages of flower bud development. These candidate genes provide new insights into the study of early-maturity mechanism and offer potential genetic improvement of cotton.

基于两个棉花优良品种RIL群体的早熟相关性状qtl鉴定
背景:早熟棉花品种有潜力在更广泛的地理区域种植,在中国北至北纬46°,并通过缩短其整个生长期(WGP)来解决粮食和棉花之间的土地竞争问题。因此,研究早熟的调控机制,鉴定早熟相关基因,培育综合性早熟高产棉花品种具有重要意义。结果:本研究对两个WGP差异显著的优良品种‘SCRC19’和‘SCRC21’及其150个重组自交系(RILs)进行了重测序,在26条染色体上获得了4092677个高质量单核苷酸多态性(snp)和794个bin标记。利用794个bin标记构建了全长2213.71 cM的遗传图谱。基于该图谱,共鉴定出78个与早熟相关的QTL,包括12个WGP QTL, 4个SSP QTL, 12个SFP QTL, 3个FBP QTL, 11个NFFB QTL, 8个NFB QTL, 16个HNFFB QTL和12个ph QTL。其中一个QTL簇在Dt3上的长度为108.5 ~ 109 cM,包含39个基因,数量最多。通过功能分析,我们突出了GH_D03G1554和GH_D03G1541两个与早熟相关的候选基因,它们分别被标注为bel1样同源结构域蛋白8和同源box-leucine zipper家族蛋白。结论:我们已经确定了一个与Dt3上6个早熟相关性状相关的QTL集群。通过对候选区域基因的注释,我们鉴定出两个候选基因GH_D03G1554和GH_D03G1541,在花蕾发育的不同阶段,它们在‘SCRC21’中的表达量显著高于‘SCRC19’。这些候选基因为棉花早熟机理的研究提供了新的思路,并为棉花的遗传改良提供了潜在的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Plant Biology
BMC Plant Biology 生物-植物科学
CiteScore
8.40
自引率
3.80%
发文量
539
审稿时长
3.8 months
期刊介绍: BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.
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