A Redox-Enzyme Integrated Microbial Fuel Cell Design Using the Surface Display System in Shewanella oneidensis MR-1

IF 8.3 2区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Seungwoo Baek, Hyeryeong Lee, Yoo Seok Lee, In Seop Chang, In-Geol Choi
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引用次数: 0

Abstract

A biofuel cell is an electrochemical device using exoelectrogen or biocatalysts to transfer electrons from redox reactions to the electrodes. While wild-type microbes and natural enzymes are often employed as exoelectrogen and biocatalysts, genetically engineered or modified organisms have been developed to enhance exoelectrogen activity. Here, we demonstrated a redox-enzyme integrated microbial fuel cell (REI-MFC) design based on an exoelectrogen-enhancing strategy that reinforces the electrogenic activity of Shewanella oneidensis MR1 by displaying an extra redox enzyme on the cell surface. We constructed the cell-surface display system for Shewanella oneidensis MR-1 by porting the autotransporter of Escherichia coli into the MR-1 strain. The functionality of the display system was validated by examining the various enzymes displayed on the cell surface of S. oneidensis MR-1. The implementation of the REI-MFC design was accomplished by an engineered MR-1 strain displaying a redox enzyme originating from swine NADH-cytochrome b5 reductase 3 (B5R3). At the polarization test of enhanced exoelectrogen in an operating MFC environment, the current generation (ΔIa, peak: 10.4 ± 1.9 μA) of the MR-1 displaying B5R3 was 4.7-fold higher than that of wild-type MR-1 (2.2 ± 0.3 μA). The maximum charge transfer resistance (Rct) under the optimized electrochemical test conditions was 70% lower than the wild-type MR-1. The cell surface display system for S. oneidensis MR-1 exploited in this study facilitated the exoelectrogen activity in the REI-MFC design.

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来源期刊
ACS Applied Materials & Interfaces
ACS Applied Materials & Interfaces 工程技术-材料科学:综合
CiteScore
16.00
自引率
6.30%
发文量
4978
审稿时长
1.8 months
期刊介绍: ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.
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