Agropine-type rolA modulates ROS homeostasis in an auxin-dependent manner in rolA-expressing cell cultures of Rubia cordifolia L.

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-12-23 DOI:10.1007/s00425-024-04597-7
Galina N Veremeichik, Taisia O Solomatina, Anastasia A Khopta, Evgenia V Brodovskaya, Tatiana Yu Gorpenchenko, Valeria P Grigorchuk, Dmitrii V Bulgakov, Victor P Bulgakov
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引用次数: 0

Abstract

Main conclusion: Long-term cultured calli may experience a biosynthetic shift due to the IAA-dependent expression of the rolA gene, which also affects ROS metabolism. The "hairy root" syndrome is caused by the root-inducing Ri-plasmid of Rhizobium rhizogenes, also known as Agrobacterium rhizogenes. The Ri-plasmid contains genes known as rol genes or root oncogenic loci, which promote root development. The important implications of the rolA gene from the T-DNA include reduced plant size, resistance to infections, and the activation of specialised metabolism. Nevertheless, rolA does not belong to the plast gene group because its function is still uncertain. Recent investigations have shown two important effects of the rolA gene. First, the production of secondary metabolites has changed in long-term cultivated rolA-transgenic calli of Rubia cordifolia L. Second, the expression of both the rolA and rolB genes has a strong auxin-dependent antagonistic effect on reactive oxygen species (ROS) homeostasis. In this work, we attempted to elucidate two rolA gene phenomena: what caused the secondary metabolism of long-term cultured calli to change? How does the individual expression of the rolA gene affect ROS homeostasis? We analysed SNPs in the 5' untranslated region and coding region of the rolA gene. These mutations do not affect the known essential amino acids of the RolA proteins. Notably, in the promoter of the rolA gene, an ACTTTA motif for auxin-mediated transcription factors was identified. Using two separate cell cultures, we demonstrated the strong auxin dependence of rolA gene expression. The expression of genes involved in ROS metabolism decreased in response to an auxin-mediated increase in rolA gene expression. Two assumptions can be made. The long-term cultivation of calli may cause changes in the hormonal state of the culture over time, which may modulate the action of the RolA protein. Moreover, auxin-dependent expression of the rolA gene led to a decrease in ROS metabolism. It can be assumed that the antagonistic interaction between rolA and rolB prevents strong rolB-induced auxin sensitivity and oxidative bursts to balance the cell state.

在表达rolA的Rubia cordifolia L细胞培养中,agropine型rolA以生长素依赖的方式调节ROS稳态。
主要结论:长期培养的愈伤组织可能由于iaa依赖性的rolA基因表达而发生生物合成转变,这也影响了ROS的代谢。“毛状根”综合征是由根生根瘤菌(也称为根生农杆菌)诱导根的ri质粒引起的。ri质粒含有促进根发育的rol基因或根致癌位点。来自T-DNA的rolA基因的重要含义包括缩小植物大小,抵抗感染,以及激活专门的代谢。然而,由于其功能尚不明确,因此不属于质体基因群。最近的研究显示了罗拉基因的两个重要作用。首先,在长期培养的转rolA基因的鲁柏愈伤组织中,次生代谢产物的产生发生了变化;其次,rolA和rolB基因的表达对活性氧(ROS)稳态具有很强的生长素依赖性拮抗作用。在这项工作中,我们试图阐明两个rolA基因现象:是什么导致了长期培养的愈伤组织的次生代谢发生变化?rolA基因的个体表达如何影响ROS稳态?我们分析了rolA基因5'非翻译区和编码区的snp。这些突变不影响已知的RolA蛋白必需氨基酸。值得注意的是,在rolA基因的启动子中,发现了生长素介导的转录因子的ACTTTA基序。通过两个独立的细胞培养,我们证明了rolA基因表达对生长素的强烈依赖性。生长素介导的rolA基因表达增加导致参与ROS代谢的基因表达减少。可以做两个假设。愈伤组织的长期培养可能引起愈伤组织的激素状态随着时间的推移而发生变化,这可能会调节RolA蛋白的作用。此外,生长素依赖性的rolA基因表达导致ROS代谢减少。可以假设,rolA和rolB之间的拮抗相互作用阻止了rolB诱导的强生长素敏感性和氧化爆发来平衡细胞状态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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