Fast High-Resolution Metabolite Mapping in the rat Brain Using 1H-FID-MRSI at 14.1 T.

IF 2.7 4区 医学 Q2 BIOPHYSICS
Dunja Simicic, Brayan Alves, Jessie Mosso, Guillaume Briand, Thanh Phong Lê, Ruud B van Heeswijk, Jana Starčuková, Bernard Lanz, Antoine Klauser, Bernhard Strasser, Wolfgang Bogner, Cristina Cudalbu
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引用次数: 0

Abstract

Magnetic resonance spectroscopic imaging (MRSI) enables the simultaneous noninvasive acquisition of MR spectra from multiple spatial locations inside the brain. Although 1H-MRSI is increasingly used in the human brain, it is not yet widely applied in the preclinical setting, mostly because of difficulties specifically related to very small nominal voxel size in the rat brain and low concentration of brain metabolites, resulting in low signal-to-noise ratio (SNR). In this context, we implemented a free induction decay 1H-MRSI sequence (1H-FID-MRSI) in the rat brain at 14.1 T. We combined the advantages of 1H-FID-MRSI with the ultra-high magnetic field to achieve higher SNR, coverage, and spatial resolution in the rat brain and developed a custom dedicated processing pipeline with a graphical user interface for Bruker 1H-FID-MRSI: MRS4Brain toolbox. LCModel fit, using the simulated metabolite basis set and in vivo measured MM, provided reliable fits for the data at acquisition delays of 1.30 ms. The resulting Cramér-Rao lower bounds were sufficiently low (< 30%) for eight metabolites of interest (total creatine, N-acetylaspartate, N-acetylaspartate + N-acetylaspartylglutamate, total choline, glutamine, glutamate, myo-inositol, and taurine), leading to highly reproducible metabolic maps. Similar spectral quality and metabolic maps were obtained with one and two averages, with slightly better contrast and brain coverage due to increased SNR in the latter case. Furthermore, the obtained metabolic maps were accurate enough to confirm the previously known brain regional distribution of some metabolites. The acquisitions proved high reproducibility over time. We demonstrated that the increased SNR and spectral resolution at 14.1 T can be translated into high spatial resolution in 1H-FID-MRSI of the rat brain in 13 min using the sequence and processing pipeline described herein. High-resolution 1H-FID-MRSI at 14.1 T provided robust, reproducible, and high-quality metabolic mapping of brain metabolites with minimal technical limitations.

在14.1 T时使用1H-FID-MRSI快速高分辨率绘制大鼠脑代谢物图谱。
磁共振波谱成像(MRSI)能够同时从大脑内部的多个空间位置无创地获取磁共振波谱。虽然1H-MRSI在人脑中的应用越来越多,但尚未广泛应用于临床前环境,主要是因为大鼠大脑中非常小的名义体素尺寸和低浓度的脑代谢物,导致低信噪比(SNR)。在这种情况下,我们在14.1 T的大鼠脑中实施了自由诱导衰变1H-MRSI序列(1H-FID-MRSI)。我们将1H-FID-MRSI与超高磁场的优势结合起来,在大鼠大脑中实现更高的信噪比、覆盖范围和空间分辨率,并为Bruker 1H-FID-MRSI: MRS4Brain工具箱开发了一个定制的专用处理管道,带有图形用户界面。LCModel拟合使用模拟的代谢物基础集和体内测量的MM,在1.30 ms的采集延迟下为数据提供了可靠的拟合。使用本文描述的序列和处理管道,在13分钟内得到足够低的cram - rao下限(1H-FID-MRSI)。14.1 T的高分辨率1H-FID-MRSI以最小的技术限制提供了可靠、可重复和高质量的脑代谢物代谢图谱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
NMR in Biomedicine
NMR in Biomedicine 医学-光谱学
CiteScore
6.00
自引率
10.30%
发文量
209
审稿时长
3-8 weeks
期刊介绍: NMR in Biomedicine is a journal devoted to the publication of original full-length papers, rapid communications and review articles describing the development of magnetic resonance spectroscopy or imaging methods or their use to investigate physiological, biochemical, biophysical or medical problems. Topics for submitted papers should be in one of the following general categories: (a) development of methods and instrumentation for MR of biological systems; (b) studies of normal or diseased organs, tissues or cells; (c) diagnosis or treatment of disease. Reports may cover work on patients or healthy human subjects, in vivo animal experiments, studies of isolated organs or cultured cells, analysis of tissue extracts, NMR theory, experimental techniques, or instrumentation.
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