To label or not: the need for validation in label-free imaging.

IF 3 3区 医学 Q2 BIOCHEMICAL RESEARCH METHODS
Journal of Biomedical Optics Pub Date : 2024-06-01 Epub Date: 2024-12-20 DOI:10.1117/1.JBO.29.S2.S22717
Joseph M Szulczewski, Filiz Yesilkoy, Tyler K Ulland, Randy Bartels, Bryan A Millis, Stephen A Boppart, Kevin W Eliceiri
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引用次数: 0

Abstract

Significance: Advances in label-free imaging have impacted many areas of biological and biomedical imaging ranging from cell biology and cancer to pathology and neuroscience. Despite the great progress and advantages of these methods, it is clear that to realize their full potential, validation by extrinsic labels and probes is critically needed.

Aim: This perspective calls for developing and applying innovative labels and probes to validate both existing and emerging label-free imaging methods.

Approach: Major representative types of label-free imaging methods are briefly presented discussing their advantages and differing contrasts. Their biological applications are also reviewed with a focus on how validation of label-free methods with carefully developed labeling approaches will greatly aid in further intrinsic contrast imaging adoption and likely lead to more sophisticated image-based biomarkers and a better understanding of the underlying signals.

Conclusions: Expanded efforts in extrinsic label validation will significantly push forward the utilization and adoption of label-free methods both in basic research and clinical models.

标签或不标签:需要验证在无标签成像。
意义:无标签成像技术的进步影响了生物和生物医学成像的许多领域,从细胞生物学和癌症到病理学和神经科学。尽管这些方法有很大的进步和优势,但很明显,为了充分发挥它们的潜力,迫切需要外部标签和探针的验证。目的:这一观点要求开发和应用创新的标签和探针来验证现有的和新兴的无标签成像方法。方法:简要介绍了无标记成像的主要代表类型,讨论了它们的优点和不同的对比。它们的生物学应用也进行了回顾,重点是如何验证无标记方法与精心开发的标记方法将极大地帮助进一步采用内在对比成像,并可能导致更复杂的基于图像的生物标志物和更好地理解潜在的信号。结论:扩大外部标签验证的力度将显著推动无标签方法在基础研究和临床模型中的应用和采用。
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来源期刊
CiteScore
6.40
自引率
5.70%
发文量
263
审稿时长
2 months
期刊介绍: The Journal of Biomedical Optics publishes peer-reviewed papers on the use of modern optical technology for improved health care and biomedical research.
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