Comprehensive mapping of molecular cytogenetic markers in pitaya (Hylocereus undatus) and related species.

IF 4.1 2区 生物学 Q1 PLANT SCIENCES
Frontiers in Plant Science Pub Date : 2024-12-06 eCollection Date: 2024-01-01 DOI:10.3389/fpls.2024.1493776
Arrashid Harun, Shipeng Song, Xixi You, Hui Liu, Xiaopeng Wen, Zhongming Fang, Zhihao Cheng, Chunli Chen
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引用次数: 0

Abstract

Pitaya (Hylocereus undatus; 2n=22) is an important fruit crop from the Cactaceae family, originally domesticated in Mexico and the USA, and is now widely cultivated for its nutritional benefits. It is characterized by its distinctive triangular-shaped stems and large, showy flowers, thriving in arid and semi-arid environments, particularly in hot, dry climates. However, systematic chromosomal studies, including chromosomal mapping of cytogenetic markers in pitaya, are limited, presenting challenges for its cytogenetic improvement. To address this issue, we designed oligo-barcodes specific to thirty-three chromosome regions based on the pitaya reference genome and applied them to both pitaya and cactus (Selenicerus grandifloras; 2n=22) for oligo-barcodes mapping, karyotyping, and chromosome identification. We utilized FISH technology, employing oligo, rDNA, and tandem repeat probes for chromosomal mapping, identification, and karyotyping of pitaya and related species. We successfully localized oligo-barcodes on eleven pairs of chromosomes in both pitaya and cactus, demonstrating the effectiveness of the synthesized oligo-barcodes. We used two ribosomal DNA (rDNA) probes (45S and 5S) and two tandem repeat probes (GTR11 and STR3) in pitaya (both diploid and tetraploid) and two other Cactaceae species (S. grandifloras and Opuntia humifusa; 2n=40) for chromosomal mapping. The analysis of rDNA distribution and CMA (Chromomycin A3) banding across different chromosomes in pitaya and cacti highlights the concept of conserved rDNA. This study provides fundamental insights into cytogenetic markers and their localization across different chromosomes in pitaya and other Cactaceae species.

火龙果(Hylocereus undatus)及其近缘种分子细胞遗传学标记的综合定位。
火龙果(Hylocereus undatus;仙人掌(2n=22)是仙人掌科的一种重要水果作物,最初在墨西哥和美国被驯化,现在因其营养价值而被广泛种植。它的特点是其独特的三角形茎和大而艳丽的花,在干旱和半干旱的环境中,特别是在炎热干燥的气候中茁壮成长。然而,系统的染色体研究,包括对火龙果细胞遗传标记的染色体定位,是有限的,为其细胞遗传学的改进提出了挑战。为了解决这一问题,我们基于火龙果参考基因组设计了33个染色体区域的寡核苷酸条形码,并将其应用于火龙果和仙人掌(Selenicerus citifloras;2n=22)用于寡核苷酸条形码定位、核型和染色体鉴定。我们利用FISH技术,利用寡核苷酸、rDNA和串联重复序列探针对火龙果及其相关物种进行染色体定位、鉴定和核型分析。我们成功地在火龙果和仙人掌的11对染色体上定位了寡核苷酸条形码,证明了合成的寡核苷酸条形码的有效性。我们使用两个核糖体DNA (rDNA)探针(45S和5S)和两个串列重复序列探针(GTR11和STR3)对火龙果(二倍体和四倍体)和其他两种仙人掌科植物(S.桔梗和Opuntia humifusa)进行了检测;2n=40)进行染色体作图。对火龙果和仙人掌不同染色体rDNA分布和CMA (Chromomycin A3)带带的分析突出了rDNA保守的概念。该研究为火龙果和其他仙人掌科植物的细胞遗传学标记及其在不同染色体上的定位提供了基本的见解。
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来源期刊
Frontiers in Plant Science
Frontiers in Plant Science PLANT SCIENCES-
CiteScore
7.30
自引率
14.30%
发文量
4844
审稿时长
14 weeks
期刊介绍: In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches. Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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