Activity of extracts and isolated compounds Trichilia catigua against clinically relevant candida species.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2024-12-22 DOI:10.1080/17460913.2024.2444163

Mariane Roberta Ritter, Daniella Renata Faria, Franciele Abigail Vilugron Rodrigues, Danielle Lazarin-Bidóia, Daniela Cristina de Medeiros Araújo, Flavio Augusto Vicente Seixas, Érika Seki Kioshima, João Carlos Palazzo de Mello

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引用次数: 0

Abstract

Objectives: To evaluate the in vitro antifungal activity of extracts and compounds from Trichilia catigua against clinically relevant Candida species, notably Candida glabrata, and investigate possible mechanisms of action using electron microscopy and in silico techniques.

Methods: Extracts and fractions of T. catigua were obtained through turboextraction and partitioning, while the isolated compounds were previously purified. The ethyl acetate fraction (EAF) was characterized by HPLC. Antifungal activity against C. glabrata was evaluated through MIC tests, synergism was assessed via checkerboard assays, and structural changes were analyzed via electron microscopy. Molecular docking was performed to identify potential targets of action.

Results: The EAF and isolated compounds (cinchonains and procyanidin B2) exhibited significant activity against C. glabrata, with MICs of 9.76 µg/mL (EAF) and 3.9 µg/mL (cinchonains Ia and Ib). Cinchonain Ib combined with epicatechin or procyanidin B2 displayed synergistic effects, particularly with amphotericin B. Microscopy analysis revealed cell membrane damage, and reverse docking analysis suggested that the compounds may target an enzyme essential to the metabolic processes of C. glabrata.

Conclusions: The findings suggest that compounds isolated from T. catigua hold considerable potential for developing new antifungal agents against Candida species, particularly C. glabrata, with promising safety and synergistic profiles.

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.