Activity of extracts and isolated compounds Trichilia catigua against clinically relevant candida species.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2025-02-01 Epub Date: 2024-12-22 DOI:10.1080/17460913.2024.2444163

Mariane Roberta Ritter, Daniella Renata Faria, Franciele Abigail Vilugron Rodrigues, Danielle Lazarin-Bidóia, Daniela Cristina de Medeiros Araújo, Flavio Augusto Vicente Seixas, Érika Seki Kioshima, João Carlos Palazzo de Mello

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引用次数: 0

Abstract

Objectives: To evaluate the in vitro antifungal activity of extracts and compounds from Trichilia catigua against clinically relevant Candida species, notably Candida glabrata, and investigate possible mechanisms of action using electron microscopy and in silico techniques.

Methods: Extracts and fractions of T. catigua were obtained through turboextraction and partitioning, while the isolated compounds were previously purified. The ethyl acetate fraction (EAF) was characterized by HPLC. Antifungal activity against C. glabrata was evaluated through MIC tests, synergism was assessed via checkerboard assays, and structural changes were analyzed via electron microscopy. Molecular docking was performed to identify potential targets of action.

Results: The EAF and isolated compounds (cinchonains and procyanidin B2) exhibited significant activity against C. glabrata, with MICs of 9.76 µg/mL (EAF) and 3.9 µg/mL (cinchonains Ia and Ib). Cinchonain Ib combined with epicatechin or procyanidin B2 displayed synergistic effects, particularly with amphotericin B. Microscopy analysis revealed cell membrane damage, and reverse docking analysis suggested that the compounds may target an enzyme essential to the metabolic processes of C. glabrata.

Conclusions: The findings suggest that compounds isolated from T. catigua hold considerable potential for developing new antifungal agents against Candida species, particularly C. glabrata, with promising safety and synergistic profiles.

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毛毛菌提取物及分离化合物对临床相关念珠菌的活性研究。

目的：利用电子显微镜和计算机成像技术，评价毛纤毛菌提取物和化合物对临床相关念珠菌（特别是光念珠菌）的体外抗真菌活性，并探讨其可能的作用机制。方法：采用涡轮萃取和分馏的方法，分离得到的化合物经预先纯化。采用高效液相色谱法对乙酸乙酯组分进行了表征。通过MIC试验评估其抗真菌活性，通过棋盘格实验评估其协同作用，并通过电子显微镜分析其结构变化。进行分子对接以确定潜在的作用靶点。结果：EAF和分离得到的化合物（金鸡酰胺和原花青素B2）对光棘球蛾具有显著的抗虫活性，其mic值分别为9.76µg/mL （EAF）和3.9µg/mL（金鸡酰胺Ia和Ib）。金鸡苷Ib与表儿茶素或原花青素B2联合后表现出协同作用，特别是与两性霉素b。显微镜分析显示细胞膜受损，反向对接分析表明，这些化合物可能靶向一种对光斑草代谢过程至关重要的酶。结论：从catigua中分离得到的化合物具有开发抗假丝酵母（Candida），特别是光丝酵母（C. glabrata）的潜力，具有良好的安全性和协同作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.