The Combination of Super-Active Platelet Lysate and Acellular Amniotic Membrane Enhances Endometrial Receptivity, While Simultaneously Facilitating Endometrial Repair in Rats.

IF 4.2 2区 医学 Q2 IMMUNOLOGY
Journal of Inflammation Research Pub Date : 2024-12-17 eCollection Date: 2024-01-01 DOI:10.2147/JIR.S483446
Huaying Wu, Yi Zhang, Chunxiang Liu, Xiaohan Tang, Liqun Wang, Lingqi Meng, Meisong Lu
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Abstract

Purpose: To investigate the combined effects of super-active platelet lysate (sPL) and acellular amniotic membrane (AAM) in promoting endometrial repair and enhancing endometrial receptivity in rats.

Methods: The characteristics of sPL-AAM were examined through scanning electron microscopy, contact angle tester, and release experiments. We aimed to establish a rat model for endometrial injury. We divided sixty-four rats into four groups: the Injury group (Control group), the AAM group, the sPL group, and the sPL-AAM group. Our study compared the endometrial thickness, gland count, and fibrotic area recovery in rats at 6 days and 18 days post-treatment. Immunohistochemistry was utilized to assess the expressions of CD34 and ANG. Additionally, we used ELISA to detect the levels of IL-6 and TNF-α, while Western Blot was employed to compare the expressions of CK19, Integrin β3, and TGF-β1. One month after the treatment, we evaluated and compared the pregnancy recovery among the groups.

Results: Compared to the Injury group, the sPL-AAM group demonstrated enhanced endometrial regeneration in rats at both 6 days and 18 days post-treatment, resulting in a favorable pregnancy outcome. This was achieved by promoting angiogenesis, suppressing the inflammatory response, and reducing fibrosis. The observed effects were superior to those of the sPL group alone. While sPL, when administered alone, showed some degree of endometrial restoration at 6 days post-treatment, its efficacy was diminished at 18 days post-treatment. The impact of AAM alone appeared inconspicuous compared to the injury group. This suggests that sPL serves as the primary agent in facilitating endometrial repair, while AAM functions as a carrier to extend the duration of sPL's effectiveness.

Conclusion: sPL-AAM can release effective cytokines, repair endometrial damage in rats, enhance endometrial receptivity, and ultimately improve pregnancy outcomes.

超活性血小板裂解液与脱细胞羊膜联合使用可增强大鼠子宫内膜容受性,同时促进子宫内膜修复
目的:探讨超活性血小板裂解液(sPL)与脱细胞羊膜(AAM)联合促进大鼠子宫内膜修复和增强子宫内膜容受性的作用。方法:采用扫描电子显微镜、接触角测试仪、释放实验等方法检测spll - aam的特性。我们的目的是建立大鼠子宫内膜损伤模型。我们将64只大鼠分为4组:损伤组(对照组)、AAM组、sPL组和sPL-AAM组。我们的研究比较了大鼠在治疗后6天和18天的子宫内膜厚度、腺体计数和纤维化区域恢复情况。免疫组化检测CD34和ANG的表达。此外,我们采用ELISA法检测IL-6、TNF-α水平,Western Blot法比较CK19、Integrin β3、TGF-β1的表达。治疗1个月后,对各组妊娠恢复情况进行评估和比较。结果:与损伤组相比,sPL-AAM组在治疗后6天和18天均能增强大鼠子宫内膜再生,妊娠结局良好。这是通过促进血管生成、抑制炎症反应和减少纤维化来实现的。观察到的效果优于单用sPL组。当单独使用sPL时,在治疗后6天显示出一定程度的子宫内膜恢复,但在治疗后18天其效果减弱。与损伤组相比,AAM单独的影响不明显。这表明sPL是促进子宫内膜修复的主要药物,而AAM则是延长sPL有效性持续时间的载体。结论:spll - aam能释放有效的细胞因子,修复大鼠子宫内膜损伤,增强子宫内膜容受性,最终改善妊娠结局。
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来源期刊
Journal of Inflammation Research
Journal of Inflammation Research Immunology and Microbiology-Immunology
CiteScore
6.10
自引率
2.20%
发文量
658
审稿时长
16 weeks
期刊介绍: An international, peer-reviewed, open access, online journal that welcomes laboratory and clinical findings on the molecular basis, cell biology and pharmacology of inflammation.
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索莱宝
LPS
索莱宝
Triton X-100
索莱宝
lipopolysaccharide (LPS)
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