Study on the Mechanism of UMI-77 in the Treatment of Sepsis-Induced Acute Lung Injury Based on Transcriptomics and Metabolomics.

IF 4.2 2区 医学 Q2 IMMUNOLOGY
Journal of Inflammation Research Pub Date : 2024-12-18 eCollection Date: 2024-01-01 DOI:10.2147/JIR.S495512
Jiatian Zhang, Zhelin Xia, Cuicui Dong, Jiaqi Zhu, Hang Ni, Yubin Xu, Yinghe Xu
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引用次数: 0

Abstract

Introduction: Sepsis-induced acute lung injury (ALI), a critical sequela of systemic inflammation, often progresses to acute respiratory distress syndrome, conferring high mortality. Although UMI-77 has demonstrated efficacy in mitigating lung injury in sepsis, the molecular mechanisms underlying its action have not yet been fully elucidated.

Methods: This study aimed to delineate the mechanism by which UMI-77 counteracts sepsis-induced ALI using comprehensive transcriptomic and metabolomic analyses.

Results: UMI-77 significantly ameliorated histopathological changes in the lungs of mice with sepsis-induced ALI Transcriptomic analysis revealed that 124 differentially expressed genes were modulated by UMI-77 and were predominantly implicated in chemokine-mediated signaling pathways, apoptosis regulation, and inflammatory responses. Integrated metabolomic analysis identified Atp4a, Ido1, Ctla4, and Cxcl10 as key genes, and inosine 5'-monophosphate (IMP), thiamine monophosphate, thymidine 3',5'-cyclic monophosphate (dTMP) as key differential metabolites. UMI-77 may regulate key genes (Atp4a, Ido1, Ctla4, and Cxcl10) to affect key metabolites (IMP, thiamine monophosphate, and dTMP) and their target genes (Entpd2, Entpd1, Nt5e, and Hprt) involved in cytokine-cytokine receptor interaction, gastric acid secretion, pyrimidine, and purine metabolism in the treatment of sepsis-induced ALI.

Conclusion: UMI-77 exerts its therapeutic effect in sepsis-induced ALI through intricate modulation of pivotal genes and metabolites, thereby influencing critical biological pathways. This study lays the groundwork for further development and clinical translation of UMI-77 as a potential therapeutic agent for sepsis-associated lung injuries.

基于转录组学和代谢组学的uni -77治疗脓毒症急性肺损伤机制研究
简介:败血症引起的急性肺损伤(ALI)是全身性炎症的严重后遗症,常发展为急性呼吸窘迫综合征,死亡率高。虽然UMI-77已被证明具有减轻败血症肺损伤的功效,但其作用的分子机制尚未完全阐明。方法:本研究旨在通过综合转录组学和代谢组学分析来描述uni -77对抗败血症诱导的ALI的机制。结果:uni -77显著改善脓毒症诱导ALI小鼠肺部的组织病理学变化转录组学分析显示,124个差异表达基因被uni -77调节,主要涉及趋化因子介导的信号通路、凋亡调节和炎症反应。综合代谢组学分析确定Atp4a、Ido1、Ctla4和Cxcl10为关键基因,肌苷5′-单磷酸(IMP)、单磷酸硫胺素、胸苷3′,5′-环单磷酸(dTMP)为关键差异代谢产物。uni -77可能调节关键基因(Atp4a、Ido1、Ctla4和Cxcl10),影响脓毒症诱导ALI治疗中参与细胞因子-细胞因子受体相互作用、胃酸分泌、嘧啶和嘌呤代谢的关键代谢物(IMP、单磷酸硫胺素和dTMP)及其靶基因(Entpd2、Entpd1、Nt5e和Hprt)。结论:uni -77通过复杂调控关键基因和代谢物,从而影响关键生物学通路,发挥其治疗败血症性ALI的作用。本研究为进一步开发和临床转化uni -77作为败血症相关肺损伤的潜在治疗剂奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Inflammation Research
Journal of Inflammation Research Immunology and Microbiology-Immunology
CiteScore
6.10
自引率
2.20%
发文量
658
审稿时长
16 weeks
期刊介绍: An international, peer-reviewed, open access, online journal that welcomes laboratory and clinical findings on the molecular basis, cell biology and pharmacology of inflammation.
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