Dynamic microbial changes in exacerbation of chronic obstructive pulmonary disease.

IF 4 2区生物学 Q2 MICROBIOLOGY

Frontiers in Microbiology Pub Date : 2024-12-06 eCollection Date: 2024-01-01 DOI:10.3389/fmicb.2024.1507090

Yong Jun Choi, Hye Jung Park, Chi Young Kim, Aeri Choi, Jae Hwa Cho, Min Kwang Byun

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Abstract

Background: Microbial profiles in patients with chronic obstructive pulmonary disease (COPD) provide insights for predicting, preventing, and treating exacerbations. This study aimed to analyze the impact of microbial diversity and spectrum on COPD exacerbation.

Methods: From November 1, 2018, to May 31, 2023, we prospectively enrolled patients with stable disease (SD) and exacerbation of COPD (ECOPD). Sputum samples were collected for microbiome DNA sequencing, and amplicon sequence variants were analyzed.

Results: We collected sputum samples from 38 patients: 17 samples from patients with SD and samples from patients with ECOPD at two time points-during exacerbation (AE-1: 21 samples) and again during stabilization after 2 weeks of treatment (AE-2: 17 samples). Alpha diversity indices, specifically observed feature count and Fisher's alpha index, were significantly higher in SD (133.0 [98.0-145.0]; 17.1 [12.7-19.6]) compared to AE-1 (88.0 [72.0-125.0], p = 0.025; 10.9 [8.5-16.1], p = 0.031). The SD showed significantly higher abundances of Neisseria (linear discriminant analysis [LDA] 4.996, adj.p = 0.021), Fusobacterium (LDA 3.688, adj.p = 0.047), and Peptostreptococcus (LDA 3.379, adj.p = 0.039) at the genus level compared to AE-1. At the species level, N. perflava (LDA 5.074, adj.p = 0.010) and H. parainfluenzae (LDA 4.467, adj. p = 0.011) were more abundant in SD. Hub genera in the microbial network included Haemophilus, Granulicatella, Neisseria, Lactobacillus, and Butyrivibrio in SD and Streptococcus, Gemella, Actinomyces, Klebsiella, and Staphylococcus in AE-1.

Conclusion: COPD exacerbations are linked to changes in specific strains of normal flora. Maintaining microbial diversity and balance within the microbial network is critical for preventing and managing COPD exacerbations.

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慢性阻塞性肺疾病恶化的动态微生物变化。

背景：慢性阻塞性肺疾病（COPD）患者的微生物谱为预测、预防和治疗急性加重提供了见解。本研究旨在分析微生物多样性和谱对COPD加重的影响。方法：2018年11月1日至2023年5月31日，前瞻性纳入稳定期（SD） COPD加重（ECOPD）患者。采集痰液样本进行微生物组DNA测序，分析扩增子序列变异。结果：我们收集了38例患者的痰样本：17例SD患者和17例ECOPD患者在两个时间点-加重期间（AE-1: 21例）和治疗2 周后稳定期间（AE-2: 17例）的痰样本。Alpha多样性指数，特别是观察到的特征数和Fisher's Alpha指数在SD（133.0[98.0-145.0]）中显著升高；17.1(12.7 - -19.6))比AE-1 (88.0 [72.0 - -125.0], p = 0.025;10.9 [8.5-16.1], p = 0.031)。在属水平上，奈球菌（线性判别分析[LDA] 4.996, adj.p = 0.021）、梭杆菌（线性判别分析[LDA] 3.688, adj.p = 0.047）和胃链球菌（LDA 3.379, adj.p = 0.039）的丰度显著高于AE-1。在物种水平上，perflava （LDA 5.074, adjj .p = 0.010）和副流感嗜血杆菌（LDA 4.467, adjj .p = 0.011）在SD中的丰度较高。微生物网络中的中心属包括SD中的嗜血杆菌、肉芽菌、奈瑟菌、乳酸杆菌和丁酸弧菌，AE-1中的链球菌、Gemella、放线菌、克雷伯菌和葡萄球菌。结论：COPD加重与正常菌群特定菌株的变化有关。维持微生物多样性和微生物网络内的平衡对于预防和管理COPD恶化至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Microbiology MICROBIOLOGY-

CiteScore

7.70

自引率

9.60%

发文量

4837

审稿时长

14 weeks

期刊介绍： Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.