Phlomoides rotata adapts to low-nitrogen environments by promoting root growth and increasing root organic acid exudate.

IF 4.3 2区 生物学 Q1 PLANT SCIENCES
Jielin Zhang, Guopeng Chen, Yuan Li, Jie Zhang, Liwen Zhong, Ling Li, Shihong Zhong, Rui Gu
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Abstract

Nitrogen (N) is one of the three major elements required for plant growth and development. It is of great significance to study the effects of different nitrogen application levels on the growth and root exudates of Phlomoides rotata, and can provide a theoretical basis for its scientific application of fertilizer to increase production. In this study, Phlomoides rotata were grown under different nitrogen conditions for two months. Soil and plant analyzer development (SPAD) values, bioaccumulation, root morphology, root exudate composition, nitrogen metabolism enzyme and antioxidant enzyme activity were evaluated. The results showed that compared with CK (no N fertilizer), N2 (CO(NH2)2 80 mg/kg) and N3 (CO(NH2)2 160 mg/kg) through significantly improved the activities of nitrogen metabolism enzyme nitrite reductase (NiR), glutamate dehydrogenase (GDH) and glutamine synthetase (GS), enhanced the nitrogen metabolism process, and increased the accumulation of plant soluble sugars (SS) and soluble protein (SP), thus improving Phlomoides rotata biomass yield. After 60 days of treatment, low nitrogen (N1, CO(NH2)2 40 mg/kg) increased root length, root volume, root surface area, average root diameter, significantly increased the diversity of organic acids in root exudates, and enhanced the activity of antioxidant enzymes to adapt the nitrogen deficiency environment. This study can provide new ideas for understanding the mechanism of nitrogen tolerance in Phlomoides rotata and developing scientific fertilization management strategies for plateau plants and medicinal plants.

轮状蕨通过促进根系生长和增加根系有机酸分泌来适应低氮环境。
氮(N)是植物生长发育所需的三大元素之一。研究不同施氮水平对轮盘蕨生长和根系分泌物的影响具有重要意义,可为轮盘蕨科学施肥增产提供理论依据。在不同的氮素条件下,对轮状Phlomoides rotata进行了2个月的培养。评价土壤和植物分析发育(SPAD)值、生物积累、根系形态、根系分泌物组成、氮代谢酶和抗氧化酶活性。结果表明,与CK(无氮肥)相比,N2 (CO(NH2)2 80 mg/kg)和N3 (CO(NH2)2 160 mg/kg)通过显著提高氮素代谢酶亚硝酸盐还原酶(NiR)、谷氨酸脱氢酶(GDH)和谷氨酰胺合成酶(GS)活性,增强氮素代谢过程,增加植物可溶性糖(SS)和可溶性蛋白(SP)的积累,从而提高了轮状球藻生物量产量。处理60 d后,低氮(N1、CO(NH2)2 40 mg/kg)增加了根长、根体积、根表面积和平均根径,显著增加了根分泌物中有机酸的多样性,增强了抗氧化酶的活性,以适应缺氮环境。本研究可为进一步认识轮状榄耐氮机理,为高原植物和药用植物制定科学的施肥管理策略提供新的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Plant Biology
BMC Plant Biology 生物-植物科学
CiteScore
8.40
自引率
3.80%
发文量
539
审稿时长
3.8 months
期刊介绍: BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.
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