Fluorescence tunable carbon dots for in vitro nuclear dynamics and gastrointestinal imaging in live zebrafish and their in vivo toxicity evaluation by cardio-craniofacial disfunction assessment†

IF 5.8 3区 材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Nanoscale Pub Date : 2024-12-23 DOI:10.1039/D4NR04077E
Bijaideep Dutta, Ashwini Waghmare, Sourav Kumar Das, Yogesh Bhargava, Amit Kumar, A. K. Debnath, K. C. Barick and P. A. Hassan
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引用次数: 0

Abstract

Sub-cellular organelle anomalies are frequently observed in diseases such as cancer. Early and precise diagnosis of these alterations can be crucial for patient outcomes. However, current diagnostic tools using conventional organic dyes or metal quantum dots face limitations, including poor biocompatibility, stringent storage conditions, limited solubility in aqueous media, and slow staining speeds. These challenges underscore the need for safer, more effective diagnostic and therapeutic solutions. In these aspects, we have developed highly photostable, biocompatible, water-dispersible carbon dots (TNCDs) with an average size of 5.5 nm using tartaric acid and ethylenediamine via a hydrothermal route. The synthesized TNCDs have shown bright blue fluorescence under the irradiation of UV-light at an excitation wavelength of 365 nm. They exhibit a quantum yield (QY) of 25.1% with maximum emission at 390 nm. A nice tri-exponential fitting of the decay curve with characteristic lifetimes of 1.52 ns, 3.05 ns and 6.11 ns for TNCDs was obtained. In vitro studies demonstrated that TNCDs have high biocompatibility (20 μg ml−1) with almost 100% cell viability and excellent nucleus targeting and staining capabilities with low background interference (with 10–12 times enhancement in fluorescence intensity). Additionally, if tagged with photosensitizers or radionuclides, TNCDs can serve as therapeutic agents in photodynamic therapy against cancer cells. Importantly, TNCDs exhibited negligible toxicity in developing zebrafish even at high concentrations (up to 400 mg L−1) as investigated by cardio and craniofacial disfunction assessment. Live organism imaging revealed that TNCDs produced aggregation-induced strong and specific green fluorescence in the gut of zebrafish larvae even at low concentrations, indicating their potential for nucleus staining and gut-specific optical imaging (at 50 mg L−1). Thus, our TNCDs represent a robust nanoplatform for cellular and whole-organism fluorescence imaging, offering both diagnostic and therapeutic potential.

Abstract Image

荧光可调谐碳点用于活体斑马鱼的体外核动力学和胃肠道成像及其通过心颅面功能障碍评估的体内毒性评估。
亚细胞器异常在癌症等疾病中经常观察到。这些改变的早期和精确诊断对患者的预后至关重要。然而,目前使用传统有机染料或金属量子点的诊断工具面临着局限性,包括生物相容性差、储存条件严格、在水介质中的溶解度有限以及染色速度慢。这些挑战强调需要更安全、更有效的诊断和治疗解决方案。在这些方面,我们利用酒石酸和乙二胺通过水热途径制备了一种平均尺寸为5.5 nm的高度光稳定性、生物相容性、水分散的碳点(tncd)。合成的TNCDs在365nm激发波长的紫外光照射下显示出明亮的蓝色荧光。它的量子产率(QY)为25.1%,最大发射波长为390nm。对TNCDs的特征寿命分别为1.52 ns、3.05 ns和6.11 ns的衰减曲线进行了三指数拟合。体外研究表明,TNCDs具有高的生物相容性(20 μg/ml),几乎100%的细胞活力,出色的细胞核靶向性和低背景干扰的染色能力(荧光强度增强10-12倍)。此外,当用光敏剂或放射性核素标记时,tncd可以作为治疗药物用于对癌细胞的光动力治疗。重要的是,通过心脏和颅面功能评估,即使在高浓度(高达400 mg/L)下,tncd对发育中的斑马鱼的毒性也可以忽略不计。活体成像显示,即使在低浓度下,tncd也能在斑马鱼幼虫的肠道中产生聚集诱导的强特异性绿色荧光,表明它们具有核染色和肠道特异性光学成像的潜力(50 mg/L)。因此,我们的tncd代表了细胞和整个生物体荧光成像的强大纳米平台,提供诊断和治疗潜力。关键词:生物相容性碳点,细胞器成像,斑马鱼,毒性
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来源期刊
Nanoscale
Nanoscale CHEMISTRY, MULTIDISCIPLINARY-NANOSCIENCE & NANOTECHNOLOGY
CiteScore
12.10
自引率
3.00%
发文量
1628
审稿时长
1.6 months
期刊介绍: Nanoscale is a high-impact international journal, publishing high-quality research across nanoscience and nanotechnology. Nanoscale publishes a full mix of research articles on experimental and theoretical work, including reviews, communications, and full papers.Highly interdisciplinary, this journal appeals to scientists, researchers and professionals interested in nanoscience and nanotechnology, quantum materials and quantum technology, including the areas of physics, chemistry, biology, medicine, materials, energy/environment, information technology, detection science, healthcare and drug discovery, and electronics.
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