Nitrided Ti-6Al-4V: A Catalyst for Increase Mineralization and Osteogenic Marker Expression

IF 3.9 3区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of biomedical materials research. Part A Pub Date : 2024-12-22 DOI:10.1002/jbm.a.37853

Annop Krasaesin, Suttiporn Pinijsuwan, Chatdanai Boonruang, Kanokwan Sriwattanapong, Thantrira Porntaveetus, Thanaphum Osathanon, Shuichi Watanabe, Chavin Jongwannasiri, Chawan Manaspon

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引用次数: 0

Abstract

Plasma nitriding is one of the surface modifications that show more effectiveness than other methods. In this study, the plasma-based ion implantation (PBII) technique was performed on the surface of titanium alloy (Ti-6Al-4V, Ti64) using a mixture of nitrogen (N₂) and argon (Ar), resulting in a plasma-nitrided surface (TiN-Ti64). The surface composition of the TiN-Ti64 was verified through X-ray photoelectron spectroscopy (XPS). TiN-Ti64 demonstrated superior hydrophilicity compared with Ti64. TiN-Ti64 exhibited higher surface hardness than the original surface. The biological responses of primary human alveolar bone cells (hAVs) were observed on the TiN-Ti64, revealing greater activation of cell adhesion and spreading compared with Ti64 and the control group (glass coverslip). Moreover, the TiN-Ti64 significantly promoted cell proliferation compared with Ti64 and tissue culture plates. The mineralization of hAVs on the TiN-Ti64 showed a significant increase, almost 20% greater than that of Ti64. Furthermore, a significant upregulation of mRNA expression for osteogenic differentiation marker genes, including BMP2, OCN, OPN, and RUNX2, was observed in TiN-Ti64 compared with other conditions. In addition, the TiN-Ti64 exhibited antibiofilm activity against Streptococcus aureus. In conclusion, the TiN-Ti64, modified with the PBII technique utilizing a mixture of N₂ and Ar, emerges as a promising alternative for surface modification in dental implant applications.

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氮化Ti-6Al-4V：增加矿化和成骨标志物表达的催化剂。

等离子体氮化是一种比其他方法更有效的表面改性方法。本研究利用氮（N2）和氩（Ar）的混合物对钛合金（Ti-6Al-4V, Ti64）表面进行了等离子体离子注入（PBII）技术，得到了等离子体氮化表面（TiN-Ti64）。通过x射线光电子能谱（XPS）验证了TiN-Ti64的表面组成。TiN-Ti64的亲水性优于Ti64。TiN-Ti64的表面硬度高于原始表面。观察了原代人牙槽骨细胞（hAVs）在TiN-Ti64上的生物学反应，发现与Ti64和对照组相比，细胞粘附和扩散的激活程度更高（玻璃盖盖）。此外，与Ti64和组织培养板相比，TiN-Ti64显著促进细胞增殖。在TiN-Ti64上，hav的矿化明显增加，比Ti64高出近20%。此外，与其他条件相比，TiN-Ti64中成骨分化标志基因（包括BMP2、OCN、OPN和RUNX2）的mRNA表达显著上调。此外，TiN-Ti64对金黄色链球菌具有抗菌活性。综上所述，利用N2和Ar的混合物进行PBII技术修饰的TiN-Ti64，是一种很有前途的牙种植体表面修饰替代方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of biomedical materials research. Part A 工程技术-材料科学：生物材料

CiteScore

10.40

自引率

2.00%

发文量

135

审稿时长

3.6 months

期刊介绍： The Journal of Biomedical Materials Research Part A is an international, interdisciplinary, English-language publication of original contributions concerning studies of the preparation, performance, and evaluation of biomaterials; the chemical, physical, toxicological, and mechanical behavior of materials in physiological environments; and the response of blood and tissues to biomaterials. The Journal publishes peer-reviewed articles on all relevant biomaterial topics including the science and technology of alloys,polymers, ceramics, and reprocessed animal and human tissues in surgery,dentistry, artificial organs, and other medical devices. The Journal also publishes articles in interdisciplinary areas such as tissue engineering and controlled release technology where biomaterials play a significant role in the performance of the medical device. The Journal of Biomedical Materials Research is the official journal of the Society for Biomaterials (USA), the Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Articles are welcomed from all scientists. Membership in the Society for Biomaterials is not a prerequisite for submission.