Engineered DR/NIR dual-emission carbonized polymer dots for simultaneous tracking of lipid droplets and lysosomes

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2024-12-17 DOI:10.1016/j.saa.2024.125598

Shan Miao , Junyong Sun , Ying Li , Qiang Zhang , Hongqi Chen , Feng Gao

{"title":"Engineered DR/NIR dual-emission carbonized polymer dots for simultaneous tracking of lipid droplets and lysosomes","authors":"Shan Miao , Junyong Sun , Ying Li , Qiang Zhang , Hongqi Chen , Feng Gao","doi":"10.1016/j.saa.2024.125598","DOIUrl":null,"url":null,"abstract":"<div><div>Developing near-infrared fluorescent probes for simultaneous tracking of lipid droplets (LDs) and lysosomes is highly desirable for studying cell metabolism. In this work, deep-red/near-infrared dual-emission carbonized polymer dots (DN-CPDs) were prepared for ratiometric monitoring of the intracellular polarity. Detailed structural analysis revealed that the deep-red emission and near-infrared peak of DN-CPDs originate from the molecular state and surface state, respectively. The surface-state emission was derived from the intraparticle charge-transfer (ICT) effect of the donor-bridge-acceptor (D-π-A) structure of DN-CPDs. The obtained DN-CPDs exhibited excellent dual-labeling ability, large Stokes shifts, ratiometric polarity sensitivity, high selectivity, and satisfactory photostability. Moreover, with the polarity distinction between LDs and lysosomes, DN-CPDs nanoprobes were successfully used to observe the dynamic changes of the two aforementioned organelles during starvation-induced lipophagy and drug-induced lipophagy inhibition processes. This work not only provides a useful tool for LD-lysosome related studies but is also valuable for the preparation of CPDs with long wavelength emission.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"329 ","pages":"Article 125598"},"PeriodicalIF":4.3000,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1386142524017645","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}

引用次数: 0

Abstract

Developing near-infrared fluorescent probes for simultaneous tracking of lipid droplets (LDs) and lysosomes is highly desirable for studying cell metabolism. In this work, deep-red/near-infrared dual-emission carbonized polymer dots (DN-CPDs) were prepared for ratiometric monitoring of the intracellular polarity. Detailed structural analysis revealed that the deep-red emission and near-infrared peak of DN-CPDs originate from the molecular state and surface state, respectively. The surface-state emission was derived from the intraparticle charge-transfer (ICT) effect of the donor-bridge-acceptor (D-π-A) structure of DN-CPDs. The obtained DN-CPDs exhibited excellent dual-labeling ability, large Stokes shifts, ratiometric polarity sensitivity, high selectivity, and satisfactory photostability. Moreover, with the polarity distinction between LDs and lysosomes, DN-CPDs nanoprobes were successfully used to observe the dynamic changes of the two aforementioned organelles during starvation-induced lipophagy and drug-induced lipophagy inhibition processes. This work not only provides a useful tool for LD-lysosome related studies but is also valuable for the preparation of CPDs with long wavelength emission.

Abstract Image

查看原文本刊更多论文

设计DR/NIR双发射碳化聚合物点，用于同时跟踪脂滴和溶酶体。

开发用于同时跟踪脂滴和溶酶体的近红外荧光探针是研究细胞代谢的迫切需要。在这项工作中，制备了深红色/近红外双发射碳化聚合物点（DN-CPDs），用于细胞内极性的比例监测。详细的结构分析表明，DN-CPDs的深红色发射峰和近红外峰分别来源于分子态和表面态。表面态发射来源于DN-CPDs的供体-桥体-受体（D-π-A）结构的粒子内电荷转移（ICT）效应。所得的DN-CPDs具有优异的双标记能力、大的Stokes位移、比例极性灵敏度、高选择性和令人满意的光稳定性。此外，利用ld和溶酶体的极性差异，DN-CPDs纳米探针成功地观察了上述两种细胞器在饥饿诱导的脂噬和药物诱导的脂噬抑制过程中的动态变化。这项工作不仅为ld溶酶体的相关研究提供了一个有用的工具，而且对制备具有长波发射特性的cpd具有重要的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.