Oral epithelial dysplasia with lichenoid features shares proteomic overlap with oral epithelial dysplasia without lymphocytic immune response

IF 2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Oral Surgery Oral Medicine Oral Pathology Oral Radiology Pub Date : 2024-11-07 DOI:10.1016/j.oooo.2024.10.085

Alejandro I. Lorenzo-Pouso PhD, DDS, MSc, MPH , Elina Pérez-García DDS , Susana B. Bravo PhD , Martina Coppini DDS, MSc , Fábio França-Vieira-E-Silva DDS, MSc , Cintia M. Chamorro-Petronacci PhD, DDS , Vito Carlo Alberto Caponio PhD, DDS, MSc , María Elena Padín-Iruegas PhD, MD , Irene Lafuente-Ibañez-de-Mendoza PhD, DDS, MSc , Pilar Gándara-Vila PhD, DDS, MSc , Mario Pérez-Sayáns PhD, DDS, MSc , Andrés Blanco-Carrión PhD, MD

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引用次数: 0

Abstract

Objective

This study investigates the proteomic profiles of oral epithelial dysplasia with lichenoid features (OEDwithLF) and evaluates its relevance as a histopathological feature for lichenoid mucositis (LM) through differential proteomic characterization.

Study Design

SWATH-MS proteomic profiling was conducted on FFPE samples from 6 OEDwithLF, 5 OED cases without associated lymphocytic infiltration, and 5 LM cases. Protein expression levels were quantified and compared. In silico analysis examined the biological and molecular functions of dysregulated proteins.

Results

A total of 460 proteins were identified. Unsupervised clustering revealed significant differences between LM and OEDwithLF, with fewer differences observed between OEDwithLF and OED. Bioinformatic analysis indicated dysregulated proteins are involved in nucleic acid binding, ribosome function, and developmental biology. Key potential biomarkers include KRT17, LYSC, CAL5, and CRNN.

Conclusions

The proteomic profile of OEDwithLF is similar to OED without associated lymphocytic infiltration, but significantly different from LM. OED is relevant in lichenoid tissues, and its proteomic changes can be detected. Although OED may coexist with interface mucositis, it is not a defining feature of LM. This challenges the exclusion of epithelial dysplasia from lichenoid diagnoses. Based on this hypothesis-generating study, further investigation is warranted.

查看原文本刊更多论文

具有地衣样特征的口腔上皮异常增生与无淋巴细胞免疫反应的口腔上皮异常增生在蛋白质组学上有重叠。

目的：本研究探讨具有地衣样特征的口腔上皮异常增生（OEDwithLF）的蛋白质组学特征，并通过差异蛋白质组学特征评估其作为地衣样粘膜炎（LM）组织病理学特征的相关性。研究设计：对6例伴lf的OED、5例无相关淋巴细胞浸润的OED和5例LM的FFPE样本进行SWATH-MS蛋白质组学分析。定量比较蛋白表达水平。计算机分析检查了失调蛋白的生物学和分子功能。结果：共鉴定出460个蛋白。无监督聚类显示LM与OEDwithLF之间存在显著差异，而OEDwithLF与OED之间的差异较小。生物信息学分析表明，失调蛋白与核酸结合、核糖体功能和发育生物学有关。关键的潜在生物标志物包括KRT17、LYSC、CAL5和CRNN。结论：伴lf的oedp蛋白组学特征与未伴淋巴细胞浸润的OED相似，但与LM有显著差异。OED与地衣样组织相关，其蛋白质组学变化可被检测到。虽然OED可能与界面粘膜炎共存，但它不是LM的定义特征。这挑战了从地衣样细胞诊断中排除上皮异常增生。基于这项产生假设的研究，进一步的调查是有必要的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral Surgery Oral Medicine Oral Pathology Oral Radiology DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

3.80

自引率

6.90%

发文量

1217

审稿时长

2-4 weeks

期刊介绍： Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology is required reading for anyone in the fields of oral surgery, oral medicine, oral pathology, oral radiology or advanced general practice dentistry. It is the only major dental journal that provides a practical and complete overview of the medical and surgical techniques of dental practice in four areas. Topics covered include such current issues as dental implants, treatment of HIV-infected patients, and evaluation and treatment of TMJ disorders. The official publication for nine societies, the Journal is recommended for initial purchase in the Brandon Hill study, Selected List of Books and Journals for the Small Medical Library.