Evaluation of the transmission of Anaplasma marginale by Amblyomma tonelliae.

IF 2 2区农林科学 Q2 PARASITOLOGY

Veterinary parasitology Pub Date : 2024-12-17 DOI:10.1016/j.vetpar.2024.110380

Matilde Nahime Mazzucco Panizza, Evelina Luisa Tarragona, Patrick Stephan Sebastian, Fernando Sebastian Flores, Atilio José Mangold, Santiago Nava

{"title":"Evaluation of the transmission of Anaplasma marginale by Amblyomma tonelliae.","authors":"Matilde Nahime Mazzucco Panizza, Evelina Luisa Tarragona, Patrick Stephan Sebastian, Fernando Sebastian Flores, Atilio José Mangold, Santiago Nava","doi":"10.1016/j.vetpar.2024.110380","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to evaluate A. marginale transstadial (TST) and transovarial transmission (TOT) by Amblyomma tonelliae through vector competence assays and analysis of natural infection in free-living ticks. This three-host tick species was chosen as model because it is a usual parasite of cattle in all their parasitic stages, making them potential vectors through TST or TOT. Experiment 1 was performed to evaluate TST of A. marginale field strains and M1 strain, and the experiment 2 to evaluate TST and TOT of A. marginale S1P strain from an experimentally acutely infected calf. Free-living larvae, nymphs and adults of A. tonelliae were analyzed to evaluate natural infection with A. marginale. One nymph pool of the experiment 2 resulted positive (minimum infection rate of 1.25 %). However, the negative results of both vector competence assays and the molecular analysis of free-living ticks indicate that the transmission of A. marginale by A. tonelliae would not occur. This finding aligns with three of the four existing studies on the TST and TOT of A. marginale by ticks of the Amblyomma genus.</p>","PeriodicalId":23716,"journal":{"name":"Veterinary parasitology","volume":"334 ","pages":"110380"},"PeriodicalIF":2.0000,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary parasitology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.vetpar.2024.110380","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

This study aimed to evaluate A. marginale transstadial (TST) and transovarial transmission (TOT) by Amblyomma tonelliae through vector competence assays and analysis of natural infection in free-living ticks. This three-host tick species was chosen as model because it is a usual parasite of cattle in all their parasitic stages, making them potential vectors through TST or TOT. Experiment 1 was performed to evaluate TST of A. marginale field strains and M1 strain, and the experiment 2 to evaluate TST and TOT of A. marginale S1P strain from an experimentally acutely infected calf. Free-living larvae, nymphs and adults of A. tonelliae were analyzed to evaluate natural infection with A. marginale. One nymph pool of the experiment 2 resulted positive (minimum infection rate of 1.25 %). However, the negative results of both vector competence assays and the molecular analysis of free-living ticks indicate that the transmission of A. marginale by A. tonelliae would not occur. This finding aligns with three of the four existing studies on the TST and TOT of A. marginale by ticks of the Amblyomma genus.

查看原文本刊更多论文

扁桃体弱视瘤传播边缘无原体的评价。

本研究旨在通过媒介能力测定和自由生活蜱自然感染情况分析，评价扁桃体双盲虫经生殖道（TST）和经生殖道（TOT）传播。之所以选择这种三宿主蜱虫作为模型，是因为它在牛的所有寄生阶段都是一种常见的寄生虫，使它们成为通过TST或TOT的潜在媒介。实验1评价了A. marginale field菌株和M1菌株的TST，实验2评价了A. marginale S1P菌株在实验性急性感染小牛身上的TST和TOT。以自由生活的tonelliae幼虫、若虫和成虫为研究对象，评价其自然侵染程度。实验2中1个若虫池阳性（最低感染率为1.25 %）。媒介能力测定和自由蜱的分子分析结果均为阴性，表明不会发生边沿蜱经舌蠓传播的情况。这一发现与现有的四项研究中的三项一致，这些研究是关于A. marginale的TST和TOT由Amblyomma属蜱进行的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary parasitology 农林科学-寄生虫学

CiteScore

5.30

自引率

7.70%

发文量

126

审稿时长

36 days

期刊介绍： The journal Veterinary Parasitology has an open access mirror journal,Veterinary Parasitology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. This journal is concerned with those aspects of helminthology, protozoology and entomology which are of interest to animal health investigators, veterinary practitioners and others with a special interest in parasitology. Papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites in all domesticated animals, fall within the scope of the journal. Papers of geographically limited (local) interest which are not of interest to an international audience will not be accepted. Authors who submit papers based on local data will need to indicate why their paper is relevant to a broader readership. Parasitological studies on laboratory animals fall within the scope of the journal only if they provide a reasonably close model of a disease of domestic animals. Additionally the journal will consider papers relating to wildlife species where they may act as disease reservoirs to domestic animals, or as a zoonotic reservoir. Case studies considered to be unique or of specific interest to the journal, will also be considered on occasions at the Editors'' discretion. Papers dealing exclusively with the taxonomy of parasites do not fall within the scope of the journal.