A salt-tolerant growth-promoting phyllosphere microbial combination from mangrove plants and its mechanism for promoting salt tolerance in rice.

IF 13.8 1区 生物学 Q1 MICROBIOLOGY
Xiangxia Yang, Rongwei Yuan, Shuangyu Yang, Zhian Dai, Na Di, Haijun Yang, Zhili He, Mi Wei
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Abstract

Background: Mangrove plants growing in the high salt environment of coastal intertidal zones colonize a variety of microorganisms in the phyllosphere, which have potential salt-tolerant and growth-promoting effects. However, the characteristics of microbial communities in the phyllosphere of mangrove species with and without salt glands and the differences between them remain unknown, and the exploration and the agricultural utilization of functional microbial resources from the leaves of mangrove plants are insufficient.

Results: In this study, we examined six typical mangrove species to unravel the differences in the diversity and structure of phyllosphere microbial communities between mangrove species with or without salt glands. Our results showed that a combination of salt-tolerant growth-promoting strains of Pantoea stewartii A and Bacillus marisflavi Y25 (A + Y25) was constructed from the phyllosphere of mangrove plants, which demonstrated an ability to modulate osmotic substances in rice and regulate the expression of salt-resistance-associated genes. Further metagenomic analysis revealed that exogenous inoculation with A + Y25 increased the rice rhizosphere's specific microbial taxon Chloroflexi, thereby elevating microbial community quorum sensing and ultimately enhancing ionic balance and overall microbial community function to aid salt resistance in rice.

Conclusions: This study advances our understanding of the mutualistic and symbiotic relationships between mangrove species and their phyllosphere microbial communities. It offers a paradigm for exploring agricultural beneficial microbial resources from mangrove leaves and providing the potential for applying the salt-tolerant bacterial consortium to enhance crop adaptability in saline-alkaline land. Video Abstract.

红树林促盐层微生物组合及其促进水稻耐盐的机制。
背景:生长在滨海潮间带高盐环境中的红树林植物在根层圈内定植了多种微生物,具有潜在的耐盐和促生长作用。然而,有盐腺和无盐腺的红树林种层层内微生物群落特征及其差异尚不清楚,红树林叶片功能微生物资源的开发和农业利用也不足。结果:本研究以6种典型红树林为研究对象,揭示了有无盐腺的红树林层际微生物群落多样性和结构的差异。结果表明,从红树植物层球中构建出一株促盐菌(Pantoea stewartii a)和marisflavi芽孢杆菌Y25 (a + Y25)组合,该组合具有调节水稻渗透物质和调控耐盐相关基因表达的能力。进一步的元基因组分析表明,外源接种A + Y25增加了水稻根际特定的微生物类群,从而提高了微生物群落的群体感应能力,最终增强了离子平衡和整体微生物群落功能,从而帮助水稻抗盐。结论:本研究促进了我们对红树林物种与其层际微生物群落之间的共生关系的认识。这为从红树林叶片中探索农业有益微生物资源提供了范例,并为应用耐盐细菌联合体提高作物对盐碱地的适应性提供了潜力。视频摘要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microbiome
Microbiome MICROBIOLOGY-
CiteScore
21.90
自引率
2.60%
发文量
198
审稿时长
4 weeks
期刊介绍: Microbiome is a journal that focuses on studies of microbiomes in humans, animals, plants, and the environment. It covers both natural and manipulated microbiomes, such as those in agriculture. The journal is interested in research that uses meta-omics approaches or novel bioinformatics tools and emphasizes the community/host interaction and structure-function relationship within the microbiome. Studies that go beyond descriptive omics surveys and include experimental or theoretical approaches will be considered for publication. The journal also encourages research that establishes cause and effect relationships and supports proposed microbiome functions. However, studies of individual microbial isolates/species without exploring their impact on the host or the complex microbiome structures and functions will not be considered for publication. Microbiome is indexed in BIOSIS, Current Contents, DOAJ, Embase, MEDLINE, PubMed, PubMed Central, and Science Citations Index Expanded.
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