Comparative assessment of morphological, cytological, and photosynthetic characteristics of the induced octoploid and its tetraploid counterpart of Celosia argentea L.

IF 4.3 2区 生物学 Q1 PLANT SCIENCES
Rohit Bharati, Yamen Homaidan Shmeit, Jana Hanzal Šedivá, Tomáš Thanh Nguyen Cong, Jiban Kumar Kundu, Lucie Severová, Roman Svoboda, Eloy Fernández-Cusimamani
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引用次数: 0

Abstract

Background: Celosia argentea is a widely recognized plant for its ornamental qualities and therapeutic uses in traditional medicine. As demand for such multipurpose plants grows, enhancing its phenotypic and physiological traits could further expand its commercial potential. Polyploidization, particularly through chemical treatments like oryzalin, offers a method to induce genetic variation and potentially improve desirable traits in plants.

Results: Tetraploid (2n = 4×= 36) nodal segments of C. argentea were treated with oryzalin under in vitro conditions, resulting in successful induction of octoploidy (2n = 8×= 72). Flow cytometry and chromosome counting confirmed polyploidization, with the highest induction rate achieved using 40 µM oryzalin for 24 h. Comparative analyses between octoploid and tetraploid plants revealed significant differences in morphological traits, including increased stem and leaf thickness, larger leaf area, inflorescence characteristics and more compact growth in the octoploids. Additionally, octoploids exhibited enhanced chlorophyll content and altered photosynthetic characteristics, along with notable changes in stomatal size and density. Ploidy stability was maintained across generations, ensuring the heritability of the induced traits.

Conclusions: In vitro polyploidization in C. argentea led to significant phenotypic and physiological improvements, demonstrating its potential for application in ornamental horticulture and plant breeding. This research contributes to the understanding of the impact of in vitro polyploidization on plant development, offering insights for the commercial cultivation and enhancement of C. argentea.

Clinical trial number: Not applicable.

凤仙花八倍体与四倍体的形态、细胞学和光合特性比较。
背景:凤尾花是一种公认的植物,具有观赏价值和传统医学治疗用途。随着对多用途植物需求的增长,提高其表型和生理性状可以进一步扩大其商业潜力。多倍体化,特别是通过像oryzalin这样的化学处理,提供了一种诱导遗传变异和潜在地改善植物理想性状的方法。结果:四倍体(2n = 4×= 36)节段经oryzalin体外处理后,成功诱导出八倍体(2n = 8×= 72)。流式细胞术和染色体计数证实了多倍体的发生,使用40µM oryzalin处理24 h诱导率最高。八倍体和四倍体的比较分析显示,八倍体在形态特征上存在显著差异,包括茎和叶厚度增加,叶面积增大,花序特征和生长更紧密。此外,八倍体叶绿素含量增加,光合特性改变,气孔大小和密度变化显著。倍性在各代间保持稳定,确保了诱导性状的遗传性。结论:银杏离体多倍体化能显著改善银杏的表型和生理,在观赏园艺和植物育种方面具有潜在的应用价值。本研究有助于了解离体多倍体对植物发育的影响,为银杏的商业化栽培和改良提供参考。临床试验号:不适用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Plant Biology
BMC Plant Biology 生物-植物科学
CiteScore
8.40
自引率
3.80%
发文量
539
审稿时长
3.8 months
期刊介绍: BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.
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