Lactobacillus casei Zhang prevents hippocampal atrophy and cognitive impairment in rats with type 2 diabetes by regulating blood glucose levels

IF 2.7 4区医学 Q3 NEUROSCIENCES

Brain Research Pub Date : 2024-12-18 DOI:10.1016/j.brainres.2024.149407

Yue Cai , Xuxia Wang , Xi Chen , Sijie Liu , Linlin Cheng , Yan Kang , Fuchun Lin

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引用次数: 0

Abstract

Purpose

Lactobacillus casei Zhang (LCZ) has health benefits, such as the ability to improve blood glucose levels in individuals with type 2 diabetes mellitus (T2DM). However, little is known about the effects of LCZ on brain structural plasticity and cognitive function in T2DM. The aims of this study were to determine whether LCZ can prevent and alleviate brain damage and memory impairment in T2DM, and to understand the mechanisms underlying the effects of LCZ in T2DM.

Methods

Forty-one male Sprague-Dawley rats were divided into the saline control (CON, n = 14), T2DM (n = 14) and T2DM + LCZ (n = 13) groups. Magnetic resonance imaging (MRI) was used to evaluate alterations in brain structure among these three groups. The novel object recognition and Y-maze tests were conducted to assess cognitive function. Histological and immunohistochemical analysis, including Nissl staining, Golgi-Cox staining and glial fibrillary acidic protein immunostaining, were performed to explore the pathophysiological mechanisms underlying brain structural changes.

Results

T2DM rats presented hyperglycemia, cognitive decline, hippocampal atrophy, and damage to hippocampal neurons and astrocytes. Compared with those in the T2DM groups, rats in the T2DM + LCZ group presented lower blood glucose levels, better cognitive function, a larger hippocampal volume, and more normal hippocampal neurons and astrocytes. There was no significant difference in these metrics between rats in the T2DM + LCZ and CON groups.

Conclusion

Hyperglycemia-induced damage to hippocampal neurons and astrocytes may lead to hippocampal atrophy and cognitive dysfunction in T2DM. LCZ can effectively prevent this damage by regulating blood glucose levels, preventing brain atrophy and cognitive impairment in T2DM rats. These findings provide a scientific basis for the clinical application of LCZ.

Abstract Image

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干酪乳杆菌张通过调节血糖水平预防2型糖尿病大鼠海马萎缩和认知障碍。

目的：干酪乳杆菌（Lactobacillus casei Zhang， LCZ）具有健康益处，如改善2型糖尿病（T2DM）患者的血糖水平。然而，LCZ对T2DM患者大脑结构可塑性和认知功能的影响尚不清楚。本研究的目的是确定LCZ是否可以预防和减轻T2DM患者的脑损伤和记忆障碍，并了解LCZ在T2DM中的作用机制。方法：41只雄性sd大鼠分为生理盐水对照组（CON, n = 14）、T2DM组（n = 14）和T2DM + LCZ组（n = 13）。使用磁共振成像（MRI）来评估三组患者大脑结构的改变。采用新颖的物体识别和y迷宫测试来评估认知功能。采用尼氏染色、高尔基-考克斯染色、胶质纤维酸性蛋白免疫染色等组织学方法探讨脑结构变化的病理生理机制。结果：T2DM大鼠出现高血糖、认知能力下降、海马萎缩、海马神经元和星形胶质细胞损伤。与T2DM组相比，T2DM + LCZ组大鼠血糖水平更低，认知功能更好，海马体积更大，海马神经元和星形胶质细胞更正常。T2DM + LCZ组和CON组大鼠在这些指标上没有显著差异。结论：高血糖引起的海马神经元和星形胶质细胞损伤可能导致T2DM海马萎缩和认知功能障碍。LCZ可通过调节血糖水平，防止T2DM大鼠脑萎缩和认知功能障碍，有效预防这种损伤。这些发现为LCZ的临床应用提供了科学依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brain Research 医学-神经科学

CiteScore

5.90

自引率

3.40%

发文量

268

审稿时长

47 days

期刊介绍： An international multidisciplinary journal devoted to fundamental research in the brain sciences. Brain Research publishes papers reporting interdisciplinary investigations of nervous system structure and function that are of general interest to the international community of neuroscientists. As is evident from the journals name, its scope is broad, ranging from cellular and molecular studies through systems neuroscience, cognition and disease. Invited reviews are also published; suggestions for and inquiries about potential reviews are welcomed. With the appearance of the final issue of the 2011 subscription, Vol. 67/1-2 (24 June 2011), Brain Research Reviews has ceased publication as a distinct journal separate from Brain Research. Review articles accepted for Brain Research are now published in that journal.