Magnetic separation-assisted DNAzyme walker-based nanosensor for point-of-care therapeutic drug monitoring of methotrexate.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-04-01 Epub Date: 2024-12-19 DOI:10.1016/j.talanta.2024.127387

Wenqing Shao, Xiaoting Liu, Yi Zhou

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引用次数: 0

Abstract

Methotrexate (MTX) is a widely used antimetabolite drug, mainly used in the treatment of a variety of cancer. Given the low therapeutic index and significant individual variability of MTX, it was critical to perform therapeutic drug monitoring (TDM) to minimize the side effects. Here, we designed a rapid and sensitive fluorescence/colorimetric assay for the detection of MTX in diluted human serum. After the aptamer binds specifically to MTX, the Walking strand cleaves the Hairpin strand and releases a large amount of fluorescent signal, and the color of the gold nanoparticles changes after the addition of sodium chloride. The change of color could be visualized by eyes to achieve point-of-care TDM of MTX. The quantitative detection of MTX concentration is carried out through the collection of fluorescent signals or absorptions. The fluorescence method can detect MTX in the range of 0.05-1000 μM with the detection limit of 0.0243 μM, meanwhile, the colorimetric method can detect MTX in the range of 0.01-100 μM with the detection limit of 0.0097 μM. The magnetic separation-assisted DNAzyme walker-based nanosensor exhibited good sensitivity, selectivity and stability for detecting MTX in serum and achieve point-of-care methods of TDM of MTX and the nanocarriers has demonstrated significant potential for clinical application.

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磁分离辅助DNAzyme助行器纳米传感器用于甲氨蝶呤的即时治疗药物监测。

甲氨蝶呤（MTX）是一种应用广泛的抗代谢药物，主要用于治疗多种癌症。鉴于MTX治疗指数低且个体差异显著，进行治疗药物监测（TDM）以减少副作用至关重要。在这里，我们设计了一种快速、灵敏的荧光/比色法检测稀释后的人血清中的MTX。适体特异性结合MTX后，Walking链裂解Hairpin链并释放大量荧光信号，加入氯化钠后金纳米颗粒的颜色发生变化。颜色变化可通过眼睛直观观察，实现MTX的即时TDM。MTX浓度的定量检测是通过收集荧光信号或吸收来进行的。荧光法对MTX的检测范围为0.05 ~ 1000 μM，检测限为0.0243 μM；比色法对MTX的检测范围为0.01 ~ 100 μM，检测限为0.0097 μM。磁分离辅助DNAzyme助行器纳米传感器对血清中MTX的检测具有良好的灵敏度、选择性和稳定性，实现了MTX TDM的点护理方法，纳米载体具有显著的临床应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.

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