Simultaneous separation of the enantiomers of six anticoagulant rodenticides using chiral supercritical fluid chromatography-mass spectrometry.

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A Pub Date : 2025-01-25 DOI:10.1016/j.chroma.2024.465600

Ana M. Ares, José Bernal, Laura Toribio

{"title":"Simultaneous separation of the enantiomers of six anticoagulant rodenticides using chiral supercritical fluid chromatography-mass spectrometry.","authors":"Ana M. Ares, José Bernal, Laura Toribio","doi":"10.1016/j.chroma.2024.465600","DOIUrl":null,"url":null,"abstract":"<div><div>The simultaneous separation of the enantiomers of six anticoagulant rodenticides, derived from 4-hydroxycoumarin, has been studied in this work. Ten different stationary phases (zwitterionic, Pirkle-type, polysaccharides and macrocyclic antibiotics derivatives) were evaluated by using supercritical fluid chromatography coupled to two different detectors (circular dichroism and mass spectrometry-single quadrupole). The effect of the type of organic modifier and temperature on the chiral separation was investigated, and the best results were obtained with the column Regis S,S-Whelk-O1 at 25 °C when using a gradient elution program with methanol as organic modifier. Considering detection, the highest sensitivity was obtained with the single quadrupole mass spectrometry detector. Under these conditions, eighteen stereoisomers were baseline resolved within 18 min. It should be also mentioned that the columns Chiralpak AS-H, Lux Cellulose-2, Chiralpak AD and Chiralpak Zwix (+) provided also good results for the enantiomeric separation of some individual compounds.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1741 ","pages":"Article 465600"},"PeriodicalIF":3.8000,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chromatography A","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0021967324009737","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

The simultaneous separation of the enantiomers of six anticoagulant rodenticides, derived from 4-hydroxycoumarin, has been studied in this work. Ten different stationary phases (zwitterionic, Pirkle-type, polysaccharides and macrocyclic antibiotics derivatives) were evaluated by using supercritical fluid chromatography coupled to two different detectors (circular dichroism and mass spectrometry-single quadrupole). The effect of the type of organic modifier and temperature on the chiral separation was investigated, and the best results were obtained with the column Regis S,S-Whelk-O1 at 25 °C when using a gradient elution program with methanol as organic modifier. Considering detection, the highest sensitivity was obtained with the single quadrupole mass spectrometry detector. Under these conditions, eighteen stereoisomers were baseline resolved within 18 min. It should be also mentioned that the columns Chiralpak AS-H, Lux Cellulose-2, Chiralpak AD and Chiralpak Zwix (+) provided also good results for the enantiomeric separation of some individual compounds.

查看原文本刊更多论文

手性超临界流体色谱-质谱法同时分离六种抗凝血灭鼠剂对映体。

研究了从4-羟基香豆素中提取的6种抗凝血灭鼠剂对映体的同时分离。采用两种不同的检测器（圆二色和质谱-单四极杆）耦合超临界流体色谱法对10种不同的固定相（两性离子、皮尔克尔型、多糖和大环抗生素衍生物）进行了评价。考察了有机改性剂种类和温度对手性分离的影响，以甲醇为有机改性剂，采用梯度洗脱程序，在25℃条件下，采用Regis S，S- whelk - o1色谱柱，获得了最佳的分离效果。从检测角度考虑，单四极杆质谱仪灵敏度最高。在此条件下，18个立体异构体在18 min内被基线分离。另外，Chiralpak AS-H、Lux Cellulose-2、Chiralpak AD和Chiralpak Zwix（+）色谱柱对某些单体化合物的对映体分离也提供了很好的结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.