A sandwich-type electrochemiluminescence biosensor based on Ni3(HAB)2/Au@ZnNiAl-LDH/Ru@MIL-53(Al)-NH2 for ultra-sensitive detection of microRNA-155

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-12-22 DOI:10.1007/s00604-024-06870-8

Zekai Wang, Lingli Qu, Yu’e Yang, Weikang Cui, Yangyang Gu, Haibo Wang, Hongzhi Pan

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引用次数: 0

Abstract

A novel electrochemiluminescence (ECL) biosensor was developed for the ultrasensitive detection of miRNA-155, based on the synergistic combination of multifunctional nanomaterials. The biosensor employed a conductive metal–organic framework (MOF), Ni₃(HAB)₂ (HAB = hexaaminobenzene), as the substrate material. The unique π-electron conjugated structure of Ni₃(HAB)₂ endowed the biosensor with excellent electron transport properties, significantly enhancing its sensitivity. Furthermore, the innovative preparation of Au@ZnNiAl-LDH nanocomposites, characterized by a high specific surface area was employed to synergistically enhance the catalytic performance of the biosensor in conjunction with Ni₃(HAB)₂. The Au@ZnNiAl-LDH also provided stable anchoring sites for the capture unit, comprised of a DNA tetrahedron hairpin composite structure (DT-HP). Additionally, a porous aluminum-based metal–organic framework (MIL-53(Al)-NH₂) was utilized to encapsulate Ru(bpy)₃²⁺, constructing a Ru@MIL-53(Al)-NH₂ signal unit that effectively improved the stability of the ECL signal. Under optimal conditions, the ECL intensity of the biosensor exhibited a robust linear relationship with the logarithm of miRNA-155 concentration over a range 3 fM to 1 nM, achieving a detection limit as low as 0.9 fM. Moreover, the biosensor demonstrated exceptional specificity, selectivity, and stability, highlighting its significant potential for applications in bioanalysis and clinical diagnosis, particularly for the early diagnosis of tumor.

Graphical Abstract

Abstract Image

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基于Ni3(HAB)2/Au@ZnNiAl-LDH/Ru@MIL-53(Al)-NH2的三明治型电化学发光生物传感器用于超灵敏检测microRNA-155

基于多功能纳米材料的协同组合，研制了一种用于超灵敏检测miRNA-155的电化学发光（ECL）生物传感器。该生物传感器采用导电金属有机骨架（MOF） Ni3(HAB)2 （HAB =六氨基苯）作为衬底材料。Ni3(HAB)2独特的π-电子共轭结构赋予了该生物传感器优异的电子传递性能，显著提高了其灵敏度。此外，创新制备的Au@ZnNiAl-LDH纳米复合材料具有高比表面积的特点，可以与Ni3(HAB)2协同增强生物传感器的催化性能。Au@ZnNiAl-LDH还为捕获单元提供了稳定的锚定位点，捕获单元由DNA四面体发夹复合结构（DT-HP）组成。此外，利用多孔铝基金属有机骨架（MIL-53(Al)-NH2）封装Ru(bpy)32+，构建Ru@MIL-53(Al)-NH2信号单元，有效提高了ECL信号的稳定性。在最佳条件下，生物传感器的ECL强度与miRNA-155浓度的对数在3 fM至1 nM范围内呈现出良好的线性关系，检测限低至0.9 fM。此外，该生物传感器表现出了卓越的特异性、选择性和稳定性，突出了其在生物分析和临床诊断，特别是肿瘤早期诊断方面的巨大应用潜力。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.