Efficacy evaluation of edaravone by imaging of •OH in cerebral ischemia-reperfusion injury with a novel fluorescent probe

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2024-12-21 DOI:10.1016/j.snb.2024.137163

Hanyue Xiang , Ayijiang Taledaohan , Mengshan Yu , Bingxuan Huang , Kai Wang , Wenzhuo Fu , Nao Xiao , Chunlin Li , Yuji Wang

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Abstract

Hydroxyl radical (•OH) is a crucial target during the course of stroke. Here, we developed a novel, blood-brain barrier (BBB) permeable fluorescent probe BIBP-H with hydrohemicyanine moiety for intelligent detection of •OH. The hydrogen in BIBP-H is abstracted by •OH to release the caged large π-conjugation fluorophore, triggering a turn-on fluorescence response. With high selectivity, sensitivity and biocompatibility, BIBP-H can monitor both endogenous and exogenous •OH in living neuron cells, and can also visualize cerebral •OH in situ in stroke rats modeled by cerebral ischemia-reperfusion injury (CIRI). Using the probe, we further corroborated the anti-apoplexy efficacy of edaravone (EDA) to reduce •OH levels. These findings reveal that BIBP-H is a convenient and reliable probe for •OH bioimaging and stroke monitoring.

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新型荧光探针•OH显像评价依达拉奉对脑缺血再灌注损伤的疗效

羟基自由基（•OH）在脑卒中过程中是一个重要的靶点。在这里，我们开发了一种具有氢半花青碱部分的新型血脑屏障（BBB）渗透性荧光探针BIBP-H，用于智能检测•OH。BIBP-H中的氢被•OH萃取，释放笼状大π共轭荧光团，触发开启荧光响应。BIBP-H具有高选择性、高灵敏度和高生物相容性，可以监测活神经元细胞内源性和外源性•OH，也可以在脑缺血再灌注损伤（CIRI）模型脑卒中大鼠中原位观察•OH。通过探针，我们进一步证实了依达拉奉（EDA）降低•OH水平的抗卒中疗效。这些发现表明，BIBP-H是一种方便可靠的•OH生物成像和脑卒中监测探针。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.