Tubules, Rods, and Spirals: Diverse Modes of SepF-FtsZ Assembling

IF 1.6 4区 生物学 Q4 CELL BIOLOGY
Cytoskeleton Pub Date : 2024-12-19 DOI:10.1002/cm.21975
Jagrity Choudhury, Barnali N. Chaudhuri
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引用次数: 0

Abstract

Z-ring formation by FtsZ, the master assembler of the divisome, is a key step in bacterial cell division. Membrane anchoring of the Z-ring requires the assistance of dedicated Z-ring binding proteins, such as SepF and FtsA. SepF participates in bundling and membrane anchoring of FtsZ in gram-positive bacteria. We report in vitro biophysical studies of the interactions between FtsZ and a cytoplasmic component of cognate SepF from three different bacteria: Mycobacterium tuberculosis, Staphylococcus aureus, and Enterococcus gallinarum. While the cytosolic domain of SepF from M. tuberculosis is primarily a dimer, those from S. aureus and E. gallinarum polymerize to form ring-like structures. Mycobacterial SepF helps in the bundling of FtsZ filaments to form thick filaments and large spirals. On the other hand, ring-forming SepF from the Firmicutes bundle FtsZ into tubules. Our results suggest that the oligomeric form of SepF directs how it bundles FtsZ filaments.

管状、杆状和螺旋状:SepF-FtsZ组装的多种模式
分裂体的主要组装体FtsZ形成z环是细菌细胞分裂的关键步骤。z -环的膜锚定需要专用的z -环结合蛋白的帮助,如SepF和FtsA。SepF参与革兰氏阳性菌中FtsZ的捆绑和膜锚定。我们报道了FtsZ与三种不同细菌(结核分枝杆菌、金黄色葡萄球菌和鸡肠球菌)同源SepF细胞质组分之间相互作用的体外生物物理研究。虽然来自结核分枝杆菌的SepF的胞质结构域主要是二聚体,但来自金黄色葡萄球菌和鸡大肠杆菌的胞质结构域聚合形成环状结构。分枝杆菌SepF有助于FtsZ丝的捆绑形成粗丝和大螺旋。另一方面,来自厚壁菌门的环状SepF将FtsZ捆绑成小管。我们的研究结果表明,SepF的寡聚形式决定了它如何捆绑FtsZ细丝。
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来源期刊
Cytoskeleton
Cytoskeleton CELL BIOLOGY-
CiteScore
5.50
自引率
3.40%
发文量
24
审稿时长
6-12 weeks
期刊介绍: Cytoskeleton focuses on all aspects of cytoskeletal research in healthy and diseased states, spanning genetic and cell biological observations, biochemical, biophysical and structural studies, mathematical modeling and theory. This includes, but is certainly not limited to, classic polymer systems of eukaryotic cells and their structural sites of attachment on membranes and organelles, as well as the bacterial cytoskeleton, the nucleoskeleton, and uncoventional polymer systems with structural/organizational roles. Cytoskeleton is published in 12 issues annually, and special issues will be dedicated to especially-active or newly-emerging areas of cytoskeletal research.
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