Cave-dwelling phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) in Thailand: population composition and pathogen detection of Bartonella and Trypanosoma.

IF 3 2区 医学 Q1 PARASITOLOGY
Sakone Sunantaraporn, Puckavadee Somwang, Pathamet Khositharattanakool, Isaraporn Unchanam, Nattiya Saenchaiban, Wilai Wongkhut, Pinpinat Sanum, Thanapat Pataradool, Rungfar Boonserm, Jérôme Depaquit, Padet Siriyasatien
{"title":"Cave-dwelling phlebotomine sand flies (Diptera: Psychodidae: Phlebotominae) in Thailand: population composition and pathogen detection of Bartonella and Trypanosoma.","authors":"Sakone Sunantaraporn, Puckavadee Somwang, Pathamet Khositharattanakool, Isaraporn Unchanam, Nattiya Saenchaiban, Wilai Wongkhut, Pinpinat Sanum, Thanapat Pataradool, Rungfar Boonserm, Jérôme Depaquit, Padet Siriyasatien","doi":"10.1186/s13071-024-06616-8","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Leishmaniasis is an emerging vector-borne disease that occurs in Thailand. Although Leishmania (Mundinia) parasites, the causative agents of the disease have been identified, the vectors of the disease remain unidentified. In the present study, we collected sand flies from three caves located in endemic areas of leishmaniasis, including Lampang and Chiang Rai in northern Thailand, and Songkhla in southern Thailand.</p><p><strong>Methods: </strong>Female sand flies were identified on the basis of morphological characteristics and confirmed by cytochrome c oxidase subunit I (COI) sequencing. Sand fly DNA samples were screened for Leishmania, Trypanosoma, and Bartonella DNA by polymerase chain reaction (PCR) on the basis of the ITS1 region of the ribosomal RNA (rRNA), SSU rRNA, and gltA genes, followed by phylogenetic relationships and haplotype diversity analysis.</p><p><strong>Results: </strong>A total of 557 sand flies were identified, comprising four genera (Sergentomyia, Phlebotomus, Grassomyia, and Idiophlebotomus) and 11 species. Molecular detection of pathogens demonstrated that Leishmania DNA was not detected. However, Trypanosoma DNA was detected in 11 samples of Phlebotomus mascomai from Lampang (7 for T. noyesi), Se. anodontis from Chiang Rai (1 each for T. noyesi and Trypanosoma sp.), and Se. khawi from Songkhla (2 for Trypanosoma sp.). Bartonella DNA was detected in 16 samples of Se. anodontis and Se. barraudi s.l. from Chiang Rai, Se. anodontis from Lampang, and Se. khawi from Songkhla. The novel Bartonella sp. detected in Thai sand flies was phylogenetically related to Bartonella sp. from bats. Genetic diversity analysis showed high haplotype diversity in both Trypanosoma parasites and Bartonella bacteria.</p><p><strong>Conclusions: </strong>The data from the present study indicate that phlebotomine sand flies could be potential vectors of zoonotic diseases caused by Trypanosoma sp. and Bartonella sp. To our knowledge, this is the first report of the natural infection of Bartonella associated with bats in Thailand, and the presence of T. noyesi and amphibian trypanosomes. However, further investigation is required to elucidate and enhance the understanding of potential vectors and transmission dynamics of pathogens in Thailand, particularly with regard to different seasonality, habitats, and host ranges.</p>","PeriodicalId":19793,"journal":{"name":"Parasites & Vectors","volume":"17 1","pages":"523"},"PeriodicalIF":3.0000,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11661008/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Parasites & Vectors","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s13071-024-06616-8","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Leishmaniasis is an emerging vector-borne disease that occurs in Thailand. Although Leishmania (Mundinia) parasites, the causative agents of the disease have been identified, the vectors of the disease remain unidentified. In the present study, we collected sand flies from three caves located in endemic areas of leishmaniasis, including Lampang and Chiang Rai in northern Thailand, and Songkhla in southern Thailand.

Methods: Female sand flies were identified on the basis of morphological characteristics and confirmed by cytochrome c oxidase subunit I (COI) sequencing. Sand fly DNA samples were screened for Leishmania, Trypanosoma, and Bartonella DNA by polymerase chain reaction (PCR) on the basis of the ITS1 region of the ribosomal RNA (rRNA), SSU rRNA, and gltA genes, followed by phylogenetic relationships and haplotype diversity analysis.

Results: A total of 557 sand flies were identified, comprising four genera (Sergentomyia, Phlebotomus, Grassomyia, and Idiophlebotomus) and 11 species. Molecular detection of pathogens demonstrated that Leishmania DNA was not detected. However, Trypanosoma DNA was detected in 11 samples of Phlebotomus mascomai from Lampang (7 for T. noyesi), Se. anodontis from Chiang Rai (1 each for T. noyesi and Trypanosoma sp.), and Se. khawi from Songkhla (2 for Trypanosoma sp.). Bartonella DNA was detected in 16 samples of Se. anodontis and Se. barraudi s.l. from Chiang Rai, Se. anodontis from Lampang, and Se. khawi from Songkhla. The novel Bartonella sp. detected in Thai sand flies was phylogenetically related to Bartonella sp. from bats. Genetic diversity analysis showed high haplotype diversity in both Trypanosoma parasites and Bartonella bacteria.

Conclusions: The data from the present study indicate that phlebotomine sand flies could be potential vectors of zoonotic diseases caused by Trypanosoma sp. and Bartonella sp. To our knowledge, this is the first report of the natural infection of Bartonella associated with bats in Thailand, and the presence of T. noyesi and amphibian trypanosomes. However, further investigation is required to elucidate and enhance the understanding of potential vectors and transmission dynamics of pathogens in Thailand, particularly with regard to different seasonality, habitats, and host ranges.

泰国穴居白蛉蛉(双翅目:白蛉科)巴尔通体和锥虫种群组成及病原检测。
背景:利什曼病是发生在泰国的一种新出现的媒介传播疾病。虽然已查明该病的病原体利什曼原虫(蒙蒂尼亚)寄生虫,但仍未查明该病的病媒。在本研究中,我们从位于利什曼病流行地区的三个洞穴中收集沙蝇,包括泰国北部的南邦和清莱,以及泰国南部的宋卡。方法:根据形态特征对雌性沙蝇进行鉴定,并进行细胞色素c氧化酶亚基I (COI)测序。基于沙蝇核糖体RNA (rRNA)、SSU rRNA和gltA基因的ITS1区,采用聚合酶链式反应(PCR)对沙蝇样本进行利什曼原虫、锥虫和巴尔通体DNA的筛选,并进行系统发育关系和单倍型多样性分析。结果:共鉴定出沙蝇557只,包括沙蝇属、沙蝇属、沙蝇属和沙蝇属4属11种。病原分子检测显示未检出利什曼原虫DNA。然而,在南邦的11份马氏白蛉样本中检测到锥虫DNA(7份为诺伊氏白蛉)。来自清莱的口腔炎(诺伊氏绦虫和锥虫各1例);来自宋卡岛的khawi(2种锥虫)。在16份样品中检测到巴尔通体DNA。牙周炎和牙周炎。barraudi s.l来自泰国清莱。来自南邦的口腔炎;来自宋卡的khawi。在泰国沙蝇中发现的新型巴尔通体与蝙蝠的巴尔通体有亲缘关系。遗传多样性分析表明,锥虫和巴尔通体具有较高的单倍型多样性。结论:本研究结果提示白蛉可能是由锥虫和巴尔通体引起的人畜共患疾病的潜在媒介。据我们所知,这是泰国首次报道蝙蝠自然感染巴尔通体,以及诺耶西锥虫和两栖类锥虫的存在。然而,需要进一步调查以阐明和加强对泰国病原体潜在媒介和传播动态的了解,特别是在不同的季节、栖息地和宿主范围方面。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Parasites & Vectors
Parasites & Vectors 医学-寄生虫学
CiteScore
6.30
自引率
9.40%
发文量
433
审稿时长
1.4 months
期刊介绍: Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信