Concentric Ring Trajectory Sampling With k-Space Reordering Enables Assessment of Tissue-Specific T1 and T2 Relaxation for 2H-Labeled Substrates in the Human Brain at 7 T.

IF 2.7 4区 医学 Q2 BIOPHYSICS
Viola Bader, Bernhard Strasser, Wolfgang Bogner, Lukas Hingerl, Sabina Frese, Anna Duguid, Aaron Osburg, William T Clarke, Stanislav Motyka, Martin Krssak, Siegfried Trattnig, Thomas Scherer, Rupert Lanzenberger, Fabian Niess
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引用次数: 0

Abstract

Deuterium metabolic imaging (DMI) is an emerging Magnetic Resonance technique providing valuable insight into the dynamics of cellular glucose (Glc) metabolism of the human brain in vivo using deuterium-labeled (2H) glucose as non-invasive tracer. Reliable concentration estimation of 2H-Glc and downstream synthesized neurotransmitters glutamate + glutamine (Glx) requires accurate knowledge of relaxation times, but so far tissue-specific T1 and T2 relaxation times (e.g., in gray and white matter) have not been determined. Such measurements are time-consuming and particularly challenging in the presence of dynamically changing metabolite levels (e.g. 2H Glc and 2H Glx). This study aimed to assess T1 and T2 relaxation times of deuterated resonances, i.e., water, Glc and Glx in human gray and white matter using inversion recovery and Hahn spin-echo 2H MRSI (magnetic resonance spectroscopic imaging), respectively, with non-Cartesian concentric ring trajectory readout (CRT) including specific k-space reordering at 7 T. The sequence was validated using phantom measurements and all results were compared to unlocalized acquisitions. Thirteen healthy volunteers participated in the study, with 10 of them scanned ~90 min after oral administration of 0.8 g/kg [6,6'-2H]-glucose. Significantly different T1 and T2 relaxation was observed between GM and WM for 2H water (T1 GM/WM/unlocalized = 358 ± 21/328 ± 12/335 m ± 6 ms, p = 0.01) and 2H Glx (T2 GM/WM/unlocalized = 37 ± 2/35 ± 2/33 ± 3 ms, p = 0.02), respectively, consistent with unlocalized acquisitions. No significant regional differences were found for 2H water (T2 GM/WM/unlocalized = 36 ± 2/34 ± 2/31 ± 2 ms, p = 0.08), 2H Glc (T1 GM/WM/unlocalized = 70 ± 5/73 ± 4/80 ± 5 ms, p = 0.13; T2 GM/WM/unlocalized = 36 ± 1/34 ± 2/34 ± 2 ms, p = 0.24) and Glx (T1 GM/WM/unlocalized = 172 ± 15/172 ± 12/165 ± 11 ms, p = 1.00). Knowledge of tissue-specific relaxation times can enhance the accuracy of concentration estimation and metabolic flux rates in future studies, potentially improving our understanding of various brain diseases such as cancer, neurodegenerative diseases or diabetes, which are often linked to impaired glucose metabolism.

同心环轨迹采样与 k 空间重排可在 7 T 条件下评估人脑中 2H 标记基质的组织特异性 T1 和 T2 弛豫。
氘代谢成像(DMI)是一种新兴的磁共振技术,使用氘标记(2H)葡萄糖作为无创示踪剂,为体内人脑细胞葡萄糖(Glc)代谢动力学提供了有价值的见解。对2H-Glc和下游合成的神经递质谷氨酸+谷氨酰胺(Glx)的可靠浓度估计需要准确了解松弛时间,但迄今为止尚未确定组织特异性T1和T2松弛时间(例如,在灰质和白质中)。在动态变化的代谢物水平(例如2H Glc和2H Glx)存在的情况下,这种测量是耗时的,尤其具有挑战性。本研究旨在评估人类灰质和白质中氘化共振(即水、Glc和Glx)的T1和T2弛豫时间,分别使用反转恢复和Hahn自旋回波2H MRSI(磁共振光谱成像),非笛卡尔同心圆轨迹读数(CRT)包括在7 T时特定的k空间重排序。该序列使用幻影测量进行验证,并将所有结果与非定位采集进行比较。13名健康志愿者参加了这项研究,其中10名志愿者在口服0.8 g/kg [6,6'-2H]-葡萄糖后90分钟进行了扫描。2H水(T1 GM/WM/ unlocalization = 358±21/328±12/335 m±6 ms, p = 0.01)和2H Glx (T2 GM/WM/ unlocalization = 37±2/35±2/33±3 ms, p = 0.02)的T1和T2弛豫在GM和WM之间存在显著差异,与非定位获取一致。2H水(T2 GM/WM/ unlocalization = 36±2/34±2/31±2 ms, p = 0.08)、2H Glc (T1 GM/WM/ unlocalization = 70±5/73±4/80±5 ms, p = 0.13;T2通用/ WM / unlocalized = 36±1/34±1/34±2毫秒,p = 0.24)和Glx (T1通用/ WM / unlocalized = 172±15/172±15/172±11女士,p = 1.00)。组织特异性松弛时间的知识可以在未来的研究中提高浓度估计和代谢通量率的准确性,有可能提高我们对各种脑部疾病的理解,如癌症、神经退行性疾病或糖尿病,这些疾病通常与葡萄糖代谢受损有关。
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来源期刊
NMR in Biomedicine
NMR in Biomedicine 医学-光谱学
CiteScore
6.00
自引率
10.30%
发文量
209
审稿时长
3-8 weeks
期刊介绍: NMR in Biomedicine is a journal devoted to the publication of original full-length papers, rapid communications and review articles describing the development of magnetic resonance spectroscopy or imaging methods or their use to investigate physiological, biochemical, biophysical or medical problems. Topics for submitted papers should be in one of the following general categories: (a) development of methods and instrumentation for MR of biological systems; (b) studies of normal or diseased organs, tissues or cells; (c) diagnosis or treatment of disease. Reports may cover work on patients or healthy human subjects, in vivo animal experiments, studies of isolated organs or cultured cells, analysis of tissue extracts, NMR theory, experimental techniques, or instrumentation.
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