Glycosyltransferase-Mediated Modulation of Reactive Oxygen Species Enhances Non-host Resistance to Pst DC3000 in Nicotiana benthamiana.

IF 5.4 2区生物学 Q1 PLANT SCIENCES

Physiologia plantarum Pub Date : 2024-11-01 DOI:10.1111/ppl.70019

Yingjun Liu, Siyi Zhang, Min Sun, Xingqian Hao, Pinyuan Jin, Sheng Luo, Jiao Chen, Ting Zhang, Shating Ge, Huajian Zhang

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Abstract

Non-host resistance (NHR) governs defense responses against a broad range of potential pathogen species in contrast with host resistance. To identify specific genes involved in disease resistance, we used a virus-induced gene-silencing screen in Nicotiana benthamiana and identified glycosyltransferase (NbGT) as an essential component of NHR. NbGT silencing enhanced the hypersensitivity response, reactive oxygen species response, and callose deposition in N. benthamiana, improving its NHR to Pseudomonas syringae pv. tomato (Pst) DC3000. NbGT participated in reactive oxygen species accumulation caused by flg22 rather than coronatine and HrcC of Pst DC3000. Analyses of gene expression and enzyme activity demonstrated that NbGT-silenced plants exhibited enhanced expression and elevated levels of superoxide dismutase, resulting in heightened accumulation of H₂O₂. In conclusion, NbGT-silencing increases H₂O₂ accumulation by regulating superoxide dismutase activity during the immune response to flg22, enhancing resistance to Pst DC3000 in N. benthamiana. This research provides novel insights into the role of glycosyltransferases in NHR.

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来源期刊

Physiologia plantarum 生物-植物科学

CiteScore

11.00

自引率

3.10%

发文量

224

审稿时长

3.9 months

期刊介绍： Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.