Molecular and cellular mechanisms of photoperiod- and thermo-sensitive genic male sterility in plants.

Abstract

Photoperiod- and thermo-sensitive genic male sterile (P/TGMS) lines display male sterility under high-temperature/long-day light conditions and male fertility under low-temperature/short-day light conditions. P/TGMS lines are the fundamental basis for the two-line hybrid breeding, which has notably increased the yield potential and grain quality of rice cultivars. In this review, we focus on the research progress on photoperiod- and thermo-sensitive genic male sterility in plants. The essence of P/TGMS line is their ability to produce viable pollen under varying conditions. We overview the processes involved in anther and pollen development, as well as the molecular, cellular, and genetic mechanisms underlying P/TGMS in Arabidopsis, rice, and other crops. Slow development has been identified as a common mechanism of P/TGMS fertility restoration in both Arabidopsis and rice, while reactive oxygen species homeostasis has been implicated in rice P/TGMS. Furthermore, we discuss the prospective applications of P/TGMS and potential solutions to the challenges in this field. This review deepens the understanding of the mechanisms underlying P/TGMS and its utilization in two-line hybrid breeding across diverse crops.