Limonin alleviates imiquimod-induced psoriasis-like skin inflammation in mice model by downregulating inflammatory responses.

IF 3.1 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Qiang Li, Fangmei Li, Ting Wang
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Abstract

Psoriasis is a chronic inflammatory condition affecting 1-2% of the global population. Phytomedicine, which uses plant-based compounds, is emerging as a promising approach to managing such inflammatory diseases. Limonin, a phytochemical found in citrus fruits and known for its bitter taste, possesses significant pharmacological properties. In this study, we evaluated the anti-psoriatic effects of limonin using a psoriasis-induced mice model. BALB/c mice were treated with imiquimod to induce psoriasis and then administered limonin at doses of 20 and 40 mg/kg/day for 6 days. Tacrolimus ointment served as a positive control. We assessed the hematological profile to determine limonin's impact on leukocytes in the psoriasis model. Additionally, histomorphometric analysis of ear and skin tissues was conducted to evaluate the therapeutic effects of limonin. We further investigated the antioxidant properties of limonin by measuring levels of antioxidants and oxidative stress markers. The anti-inflammatory effects were evaluated by quantifying inflammatory cytokines and signaling proteins. In vitro, the cytotoxicity and anti-inflammatory potential of limonin were assessed using murine macrophage RAW264.7 cells. Our findings showed that limonin significantly reduced leukocyte counts, decreased inflammatory cell infiltration, and improved skin histoarchitecture in psoriasis-induced mice. Limonin also effectively scavenged free radicals and reduced levels of inflammatory cytokines and proteins without causing cytotoxicity in RAW264.7 cells. Overall, our in vivo and in vitro results confirm that limonin is a potent anti-inflammatory agent that effectively ameliorates imiquimod-induced psoriasis.

柠檬苦素通过下调炎症反应减轻吡喹莫德诱导的银屑病样皮肤炎症小鼠模型。
牛皮癣是一种慢性炎症性疾病,影响全球1-2%的人口。使用植物基化合物的植物医学正在成为治疗这类炎症性疾病的一种有前景的方法。柠檬苦素是一种在柑橘类水果中发现的植物化学物质,以其苦味而闻名,具有重要的药理特性。在本研究中,我们用银屑病小鼠模型来评估柠檬苦素的抗银屑病作用。用咪喹莫特诱导BALB/c小鼠牛皮癣,然后给药柠檬苦素,剂量分别为20和40 mg/kg/d,连续6 d。他克莫司软膏作为阳性对照。我们评估了血液学特征,以确定柠檬苦素对银屑病模型中白细胞的影响。此外,还对耳部和皮肤组织进行了组织形态学分析,以评价柠檬苦素的治疗效果。我们通过测定抗氧化剂和氧化应激标志物的水平进一步研究了柠檬苦素的抗氧化性能。通过量化炎症细胞因子和信号蛋白来评估抗炎作用。体外实验采用小鼠巨噬细胞RAW264.7细胞评价柠檬苦素的细胞毒性和抗炎作用。我们的研究结果表明,柠檬苦素可以显著降低银屑病小鼠的白细胞计数,减少炎症细胞浸润,改善皮肤组织结构。柠檬苦素还能有效清除自由基,降低炎症细胞因子和蛋白质水平,而不会对RAW264.7细胞产生细胞毒性。总之,我们的体内和体外实验结果证实,柠檬苦素是一种有效的抗炎剂,可以有效地改善吡喹莫德诱发的牛皮癣。
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来源期刊
CiteScore
6.20
自引率
5.60%
发文量
142
审稿时长
4-8 weeks
期刊介绍: Naunyn-Schmiedeberg''s Archives of Pharmacology was founded in 1873 by B. Naunyn, O. Schmiedeberg and E. Klebs as Archiv für experimentelle Pathologie und Pharmakologie, is the offical journal of the German Society of Experimental and Clinical Pharmacology and Toxicology (Deutsche Gesellschaft für experimentelle und klinische Pharmakologie und Toxikologie, DGPT) and the Sphingolipid Club. The journal publishes invited reviews, original articles, short communications and meeting reports and appears monthly. Naunyn-Schmiedeberg''s Archives of Pharmacology welcomes manuscripts for consideration of publication that report new and significant information on drug action and toxicity of chemical compounds. Thus, its scope covers all fields of experimental and clinical pharmacology as well as toxicology and includes studies in the fields of neuropharmacology and cardiovascular pharmacology as well as those describing drug actions at the cellular, biochemical and molecular levels. Moreover, submission of clinical trials with healthy volunteers or patients is encouraged. Short communications provide a means for rapid publication of significant findings of current interest that represent a conceptual advance in the field.
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