In vivo retinal melanin detection with the calibrated depolarization index in polarization-sensitive optical coherence tomography.

IF 3 3区 医学 Q2 BIOCHEMICAL RESEARCH METHODS
Journal of Biomedical Optics Pub Date : 2024-12-01 Epub Date: 2024-12-19 DOI:10.1117/1.JBO.29.12.126001
Mengyuan Ke, Liqin Jiang, Veluchamy A Barathi, Jocelyn Cheong, Jacqueline Chua, Leopold Schmetterer, Rainer A Leitgeb, Xinyu Liu
{"title":"<i>In vivo</i> retinal melanin detection with the calibrated depolarization index in polarization-sensitive optical coherence tomography.","authors":"Mengyuan Ke, Liqin Jiang, Veluchamy A Barathi, Jocelyn Cheong, Jacqueline Chua, Leopold Schmetterer, Rainer A Leitgeb, Xinyu Liu","doi":"10.1117/1.JBO.29.12.126001","DOIUrl":null,"url":null,"abstract":"<p><strong>Significance: </strong>A data-based calibration method with enhanced depolarization contrast in polarization-sensitive optical coherence tomography (PS-OCT) was developed and demonstrated effective for detecting melanin content in the eye.</p><p><strong>Aim: </strong>We aim to mitigate the dependence between the measured depolarization metric and the intensity signal-to-noise ratio (SNR) for improved visualization of depolarizing tissues, especially in low SNR regions, and to demonstrate the enhanced depolarization contrast to evaluate melanin presence.</p><p><strong>Approach: </strong>A function for calibrating the depolarization metric was experimentally derived from the young albino guinea pig, assuming depolarization free in the retina. A longitudinal study of guinea pigs (9 weeks) was conducted to assess the accumulation of melanin during early eye growth. Furthermore, the melanin content of the sub-macular choroid was compared in eyes with light and dark irides involving 14 human subjects in early middle adulthood.</p><p><strong>Results: </strong>We observed an increase in the improved depolarization contrast, which indicates potential melanin accumulation in the early eye development with age in the pigmented guinea pig eyes. We found a significant difference in melanin content between human eyes with light and dark colors.</p><p><strong>Conclusions: </strong>Our proposed calibration method enhanced the visualization of depolarizing structures in PS-OCT, which can be generalized to all kinds of polarization-sensitive imaging and can potentially monitor melanin in healthy and pathological eyes.</p>","PeriodicalId":15264,"journal":{"name":"Journal of Biomedical Optics","volume":"29 12","pages":"126001"},"PeriodicalIF":3.0000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11657874/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biomedical Optics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1117/1.JBO.29.12.126001","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/12/19 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

Abstract

Significance: A data-based calibration method with enhanced depolarization contrast in polarization-sensitive optical coherence tomography (PS-OCT) was developed and demonstrated effective for detecting melanin content in the eye.

Aim: We aim to mitigate the dependence between the measured depolarization metric and the intensity signal-to-noise ratio (SNR) for improved visualization of depolarizing tissues, especially in low SNR regions, and to demonstrate the enhanced depolarization contrast to evaluate melanin presence.

Approach: A function for calibrating the depolarization metric was experimentally derived from the young albino guinea pig, assuming depolarization free in the retina. A longitudinal study of guinea pigs (9 weeks) was conducted to assess the accumulation of melanin during early eye growth. Furthermore, the melanin content of the sub-macular choroid was compared in eyes with light and dark irides involving 14 human subjects in early middle adulthood.

Results: We observed an increase in the improved depolarization contrast, which indicates potential melanin accumulation in the early eye development with age in the pigmented guinea pig eyes. We found a significant difference in melanin content between human eyes with light and dark colors.

Conclusions: Our proposed calibration method enhanced the visualization of depolarizing structures in PS-OCT, which can be generalized to all kinds of polarization-sensitive imaging and can potentially monitor melanin in healthy and pathological eyes.

偏振敏感光学相干断层扫描中校正去偏振指数的体内视网膜黑色素检测。
意义:在偏振敏感光学相干断层扫描(PS-OCT)中开发了一种基于数据的校准方法,该方法具有增强的去偏振对比度,并被证明可有效检测眼睛中的黑色素含量。目的:我们的目标是减轻测量的去极化度量与强度信噪比(SNR)之间的依赖关系,以改善去极化组织的可视化,特别是在低信噪比区域,并展示增强的去极化对比度来评估黑色素的存在。方法:在假设视网膜无去极化的情况下,从幼年白化豚鼠实验推导出一个校正去极化度量的函数。对豚鼠(9周)进行了纵向研究,以评估早期眼睛生长过程中黑色素的积累。此外,我们还比较了14名中年早期受试者的浅色虹膜和深色虹膜的黄斑下脉络膜的黑色素含量。结果:我们观察到改善的去极化对比度增加,这表明随着年龄的增长,色素沉着的豚鼠眼睛在早期眼睛发育中可能存在黑色素积累。我们发现浅色和深色人眼的黑色素含量有显著差异。结论:我们提出的校正方法增强了PS-OCT去偏振结构的可视化,可推广到各种偏光敏感成像,有可能监测健康和病理眼睛的黑色素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
6.40
自引率
5.70%
发文量
263
审稿时长
2 months
期刊介绍: The Journal of Biomedical Optics publishes peer-reviewed papers on the use of modern optical technology for improved health care and biomedical research.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信