Covalent organic framework-based solid phase microextraction coupled with electrospray ionization mass spectrometry for the quantitative assessment of abnormal bile acids by triclosan exposure in mice

IF 5.6 1区 化学 Q1 CHEMISTRY, ANALYTICAL
Xinye Shi , Yuandi Xue , Yuxin Tu , Canrong Chen , Yajing Zhang , Zian Lin , Zongwei Cai
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Abstract

Bile acids, a representative diagnostic indicator of liver function, are used to visualize the extent of liver injury. Numerous studies have shown that triclosan (TCS) exposure leads to abnormal bile acid metabolism. As a result, there is a requirement to develop a fast and smart means to quantitatively monitor abnormal bile acids from exposure to triclosan in bio-sample. In this work, solid-phase microextraction (SPME) probes of sea urchin-like covalent organic frameworks (COF) were in situ synthesized on steel needles by using 1,3,5-tris(4-aminophenyl)benzene (TAPB) and 2,5-dimethoxybenzene-1,4-dicarboxaldehyde (DMTP) as two organic units and employed for extraction of bile acids. This TAPB-DMTP-COF-SPME possessed an excellent specified surface area (3351 m2 g−1) and a high regular porosity (∼3.6 nm), which was an ideal adsorbent to concentrate bile acids efficiently. The created probe, together with electrospray ionization mass spectrometry (ESI/MS), proved to be a fast and specific assay for the detection of bile acids in bio-samples. The proposed method had a low limitation of detection (0.03 μg L−1), good linearity (R2 ≥ 0.9931), wide linear range (0.10–1000.00 μg L−1) and excellent enrichment factor (63.60–252.00). Based on these excellent properties, it was successful application for the analyzing of bile acids in mice liver and feces, demonstrating the great potential of TAPB-DMTP-COF-SPME-ESI/MS in bile acids detection and liver injury diagnosis.

Abstract Image

基于共价有机骨架的固相微萃取联用电喷雾质谱法定量评价三氯生暴露小鼠胆汁酸异常。
胆汁酸是一种代表性的肝功能诊断指标,用于可视化肝损伤的程度。大量研究表明,暴露于三氯生(TCS)会导致胆汁酸代谢异常。因此,有必要开发一种快速、智能的方法来定量监测生物样品中暴露于三氯生的异常胆汁酸。本研究以1,3,5-三(4-氨基苯基)苯(TAPB)和2,5-二甲氧基苯-1,4-二甲醛(DMTP)为有机单元,在钢针上原位合成海胆样共价有机骨架(COF)固相微萃取探针,用于胆酸的提取。该TAPB-DMTP-COF-SPME具有优异的比表面积(3351 m2 g-1)和高规则孔隙率(~ 3.6 nm),是一种理想的高效浓缩胆汁酸的吸附剂。该探针与电喷雾电离质谱(ESI/MS)相结合,被证明是一种快速、特异的生物样品胆汁酸检测方法。该方法检测限低(0.03 μg -1),线性度好(R2≥0.9931),线性范围宽(0.10 ~ 1000.00 μg -1),富集系数高(63.60 ~ 252.00)。基于这些优异的性能,成功应用于小鼠肝脏和粪便中胆汁酸的分析,显示了tapb - dptp - cof - spme - esi /MS在胆汁酸检测和肝损伤诊断方面的巨大潜力。
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来源期刊
Talanta
Talanta 化学-分析化学
CiteScore
12.30
自引率
4.90%
发文量
861
审稿时长
29 days
期刊介绍: Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.
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