{"title":"A fluorescent probe based on pyrazoline with significant Stokes shifts for the detection of Cu<sup>2+</sup> ions and its applications.","authors":"Yajing Shang, Xinghu Wu, Haoting Luo","doi":"10.1039/d4ay01772b","DOIUrl":null,"url":null,"abstract":"<p><p>A simple pyrazoline derivative BBD has been synthesized for detecting Cu<sup>2+</sup> in EtOH/HEPES (v/v = 1 : 1, pH = 7). The probe has high selectivity for Cu<sup>2+</sup> by quenching the fluorescence intensity and was sensitive to pH. When excited at 398 nm, fluorescence is emitted at 520 nm with a Stokes shift of 122 nm, which is larger than that of other reported pyrazoline skeleton probes. The detection limit (LOD) of the probe for Cu<sup>2+</sup> is 0.862 μM which is lower than the value of the WHO limit (31.5 μM) in water. The 2 : 1 binding mode and probable recognition mechanism of the probe with Cu<sup>2+</sup> were confirmed by Job's plot, IR, ESI-MS, and TD-DFT analysis. Moreover, the probe has been successfully applied to actual water samples as well as cell and zebrafish imaging.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1039/d4ay01772b","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
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Abstract
A simple pyrazoline derivative BBD has been synthesized for detecting Cu2+ in EtOH/HEPES (v/v = 1 : 1, pH = 7). The probe has high selectivity for Cu2+ by quenching the fluorescence intensity and was sensitive to pH. When excited at 398 nm, fluorescence is emitted at 520 nm with a Stokes shift of 122 nm, which is larger than that of other reported pyrazoline skeleton probes. The detection limit (LOD) of the probe for Cu2+ is 0.862 μM which is lower than the value of the WHO limit (31.5 μM) in water. The 2 : 1 binding mode and probable recognition mechanism of the probe with Cu2+ were confirmed by Job's plot, IR, ESI-MS, and TD-DFT analysis. Moreover, the probe has been successfully applied to actual water samples as well as cell and zebrafish imaging.
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