Cody Pinger, Drew Porter, Bryan Cormack, Corey Fugate, Matthew Rogers
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引用次数: 0
Abstract
Total lipid content is a valuable indicator of fish health, prey quality, survival potential, stock health, and ecosystem status. Here, we demonstrate an accurate method for measuring total lipids in fish tissues using the spectrophotometric sulfo-phospho-vanillin (SPV) assay, adapted to a 96-well plate format. Samples of dried homogenate were cross-analyzed via the SPV assay and standard gravimetric lipid analysis. Initial measurements of whole fish homogenates analyzed include Pacific herring (Clupea pallasii), Pacific cod (Gadus macrocephalus), walleye pollock (G. chalcogrammus), Pacific capelin (Mallotus villosus), Chinook (Oncorhynchus tshawytscha), and coho (O. kisutch) salmon. Samples of muscle tissue were analyzed from Chinook, pink (O. gorbuscha), sockeye (O. nerka), and chum (O. keta) salmon. All SPV measurements were calibrated using menhaden oil. The mean absolute and relative difference between gravimetric and SPV analysis was 0.5 and ~ 16.4%, respectively (n = 121). To improve the accuracy of SPV assay results, linear calibration models specific to taxa and tissue matrix type were developed, enabling calculation of corrected SPV assay values. The accuracy of using these calibration models was tested by analyzing additional fish samples (n = 16). The results of the corrected SPV assay were not statistically different (p > 0.05) from gravimetric analysis for any samples measured, and the mean absolute and relative difference between the two assays improved to 0.2% and 4.6%, respectively. The SPV assay provides a rapid (2 h), high-throughput (25 samples processed in triplicate), precise (interassay coefficient of variation = 5.6%), and accurate method for quantifying the total lipid content of homogenized fish tissue.
期刊介绍：
Limnology and Oceanography: Methods (ISSN 1541-5856) is a companion to ASLO''s top-rated journal Limnology and Oceanography, and articles are held to the same high standards. In order to provide the most rapid publication consistent with high standards, Limnology and Oceanography: Methods appears in electronic format only, and the entire submission and review system is online. Articles are posted as soon as they are accepted and formatted for publication.
Limnology and Oceanography: Methods will consider manuscripts whose primary focus is methodological, and that deal with problems in the aquatic sciences. Manuscripts may present new measurement equipment, techniques for analyzing observations or samples, methods for understanding and interpreting information, analyses of metadata to examine the effectiveness of approaches, invited and contributed reviews and syntheses, and techniques for communicating and teaching in the aquatic sciences.
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