Three Fields Allelic Haplotype Frequencies of HLA-DPA1 and -DPB1 Typed by Next Generation Sequencing of 563 Families in Chinese Han Population

IF 5.9 4区医学 Q2 CELL BIOLOGY

HLA Pub Date : 2024-12-12 DOI:10.1111/tan.15810

Jian-Ling Wang, Jun-Bo He, Zhi-Min Li, Ya-Nan Zhu, DaJiang Cao, Hong-Lei Fu, Xian Zhang, Marcelo A. Fernández-Viña, Erik Rozemuller, XiangJun Liu

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引用次数: 0

Abstract

The present study investigated the HLA-DPA1 and -DPB1 allele and haplotype frequencies in the Chinese Han population. A total of 563 families, which have been typed for the purpose of haematopoietic stem cell transplantation, were included, and three-field allelic resolution typing of HLA-DPA1 and -DPB1 of all individuals were defined by the next-generation sequencing method. HLA-DPA1~DPB1 haplotypes were determined by allele segregation within a nuclear family. A total of 9 HLA-DPA1 and 39 HLA-DPB1 alleles, and 62 HLA-DPA1~DPB1 haplotypes were identified. The three most frequent alleles at HLA-DPA1 locus are HLA-DPA1*02:02:02 (46.581%), HLA-DPA1*01:03:01 (36.456%) and HLA-DPA1*02:01:01 (12.167%); and the three most frequent HLA-DPB1 alleles are HLA-DPB1*05:01:01 (34.014%), HLA-DPB1*02:01:02 (18.428%) and HLA-DPB1*04:01:01 (10.124%). The three most frequent haplotypes are HLA-DPA1*02:02:02~DPB1*05:01:01 (32.327%), HLA-DPA1*01:03:01 ~ DPB1*02:01:02 (14.654%) and HLA-DPA1*01:03:01~DPB1*04:01:01 (9.458%). The HLA-DPA1*02:01~DPB1*02:02 and HLA-DPA1*04:01~DPB1*05:01 haplotypes appear to be unique in Han Chinese. Strong global linkage disequilibrium (LD) between HLA-DPA1 and -DPB1 was confirmed (D′ = 0.848, W_n = 0.684). Moreover, the haplotype frequencies based on the family study were compared with the frequencies estimated by the expectation–maximisation algorithm, and the equivalent results were obtained except for haplotypes with very low frequencies. Our data provide a solid base for HLA-DP dimer association studies and useful information for transplantation and disease association studies.

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来源期刊

HLA Immunology and Microbiology-Immunology

CiteScore

3.00

自引率

28.80%

发文量

368

期刊介绍： HLA, the journal, publishes articles on various aspects of immunogenetics. These include the immunogenetics of cell surface antigens, the ontogeny and phylogeny of the immune system, the immunogenetics of cell interactions, the functional aspects of cell surface molecules and their natural ligands, and the role of tissue antigens in immune reactions. Additionally, the journal covers experimental and clinical transplantation, the relationships between normal tissue antigens and tumor-associated antigens, the genetic control of immune response and disease susceptibility, and the biochemistry and molecular biology of alloantigens and leukocyte differentiation. Manuscripts on molecules expressed on lymphoid cells, myeloid cells, platelets, and non-lineage-restricted antigens are welcomed. Lastly, the journal focuses on the immunogenetics of histocompatibility antigens in both humans and experimental animals, including their tissue distribution, regulation, and expression in normal and malignant cells, as well as the use of antigens as markers for disease.