{"title":"A RFLP 1-4-3 L1C Variant of PRRSV-2 Isolated in Sichuan Province, China: Genetic Characterization and Pathogenicity","authors":"Li-Shuang Deng, Zhi-Jie Jian, Yuan-Meng Wang, Bing-Zhou Huang, Tong Xu, Feng-Qin Li, Si-Yuan Lai, Yan-Ru Ai, Jian-Bo Huang, Zhi-Wen Xu, Ling Zhu","doi":"10.1155/tbed/6817783","DOIUrl":null,"url":null,"abstract":"<div>\n <p>Porcine reproductive and respiratory syndrome virus (PRRSV), known for causing reproductive disorders in sows and respiratory issues in piglets, poses a significant threat to the global swine industry. Since its initial report in 2013, the L1C (lineage 1.8/NADC30-like) PRRSV has drawn significant attention in China due to its high recombination potential and diverse pathogenicity. This study focuses on a naturally occurring recombinant L1C variant, SCABTC-202302, characterized by an restriction fragment length polymorphism (RFLP) pattern of 1-4-3. We investigate the strain’s genetic evolution, recombination, pathogenicity, and immune and antibody responses. Phylogenetic analysis of the ORF5 (open reading frame) gene classified the SCABTC-202302 strain as lineage 8.7, while whole-genome analysis categorized it as L1C. Notably, a discontinuous deletion of 131 amino acids (AAs) was observed in the NSP2 gene, along with specific AA mutations in ORF5. Recombination analysis revealed the NADC30 strain as the primary parent, with contributions from the JXA1 strain in the ORF2-ORF7 region. The strain caused lung and lymph node damage, sustained high-level viremia, and elevated inflammatory factors in infected piglets. Our study provides valuable insights into the genetic characteristics, pathogenicity, and immunological profile of L1C strains, contributing to the development of vaccines and control measures for PRRSV.</p>\n </div>","PeriodicalId":234,"journal":{"name":"Transboundary and Emerging Diseases","volume":"2024 1","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/tbed/6817783","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Transboundary and Emerging Diseases","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/tbed/6817783","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV), known for causing reproductive disorders in sows and respiratory issues in piglets, poses a significant threat to the global swine industry. Since its initial report in 2013, the L1C (lineage 1.8/NADC30-like) PRRSV has drawn significant attention in China due to its high recombination potential and diverse pathogenicity. This study focuses on a naturally occurring recombinant L1C variant, SCABTC-202302, characterized by an restriction fragment length polymorphism (RFLP) pattern of 1-4-3. We investigate the strain’s genetic evolution, recombination, pathogenicity, and immune and antibody responses. Phylogenetic analysis of the ORF5 (open reading frame) gene classified the SCABTC-202302 strain as lineage 8.7, while whole-genome analysis categorized it as L1C. Notably, a discontinuous deletion of 131 amino acids (AAs) was observed in the NSP2 gene, along with specific AA mutations in ORF5. Recombination analysis revealed the NADC30 strain as the primary parent, with contributions from the JXA1 strain in the ORF2-ORF7 region. The strain caused lung and lymph node damage, sustained high-level viremia, and elevated inflammatory factors in infected piglets. Our study provides valuable insights into the genetic characteristics, pathogenicity, and immunological profile of L1C strains, contributing to the development of vaccines and control measures for PRRSV.
期刊介绍：
Transboundary and Emerging Diseases brings together in one place the latest research on infectious diseases considered to hold the greatest economic threat to animals and humans worldwide. The journal provides a venue for global research on their diagnosis, prevention and management, and for papers on public health, pathogenesis, epidemiology, statistical modeling, diagnostics, biosecurity issues, genomics, vaccine development and rapid communication of new outbreaks. Papers should include timely research approaches using state-of-the-art technologies. The editors encourage papers adopting a science-based approach on socio-economic and environmental factors influencing the management of the bio-security threat posed by these diseases, including risk analysis and disease spread modeling. Preference will be given to communications focusing on novel science-based approaches to controlling transboundary and emerging diseases. The following topics are generally considered out-of-scope, but decisions are made on a case-by-case basis (for example, studies on cryptic wildlife populations, and those on potential species extinctions):
Pathogen discovery: a common pathogen newly recognised in a specific country, or a new pathogen or genetic sequence for which there is little context about — or insights regarding — its emergence or spread.
Prevalence estimation surveys and risk factor studies based on survey (rather than longitudinal) methodology, except when such studies are unique. Surveys of knowledge, attitudes and practices are within scope.
Diagnostic test development if not accompanied by robust sensitivity and specificity estimation from field studies.
Studies focused only on laboratory methods in which relevance to disease emergence and spread is not obvious or can not be inferred (“pure research” type studies).
Narrative literature reviews which do not generate new knowledge. Systematic and scoping reviews, and meta-analyses are within scope.
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