Synthesis, Characterization, and Functional Analysis of Mixed Manganese/Cerium Oxide/Hydroxyapatite Nanocomposites for Antibacterial Applications

IF 2.7 4区化学 Q2 CHEMISTRY, INORGANIC & NUCLEAR

Journal of Cluster Science Pub Date : 2024-12-19 DOI:10.1007/s10876-024-02750-6

Sakuntala Siri-Udom, Orrasa Prasitnok, Khongvit Prasitnok, Pongtanawat Khemthong, Chaiyasit Phawa, Wuttichai Roschat, Songkot Utara, Narid Prachumrak, Jiyapa Sripirom, Piaw Phatai

{"title":"Synthesis, Characterization, and Functional Analysis of Mixed Manganese/Cerium Oxide/Hydroxyapatite Nanocomposites for Antibacterial Applications","authors":"Sakuntala Siri-Udom, Orrasa Prasitnok, Khongvit Prasitnok, Pongtanawat Khemthong, Chaiyasit Phawa, Wuttichai Roschat, Songkot Utara, Narid Prachumrak, Jiyapa Sripirom, Piaw Phatai","doi":"10.1007/s10876-024-02750-6","DOIUrl":null,"url":null,"abstract":"<div><p>Mixed manganese/cerium oxide/hydroxyapatite composites are emerging as innovative materials with significant biomedical potential due to their antibacterial properties and biocompatibility. In this study, we synthesized mixed Mn/Ce oxide/HA composites using an ultrasonic-assisted sol-gel method, exploring their structural and functional characteristics through comprehensive analyses. Advanced characterization techniques, including X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), X-ray absorption spectroscopy (XAS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and nitrogen adsorption-desorption isotherms, revealed a composite with enhanced structural stability and porosity, optimized for antibacterial applications. XRD confirmed the integration of a fluorite-structured CeO<sub>2</sub> phase with hexagonal hydroxyapatite, while FT-IR and XANES analyses verified the presence of functional phosphate groups and mixed oxidation states (Ce<sup>3+</sup>/Ce<sup>4+</sup>, Mn<sup>2+</sup>/Mn<sup>3+</sup>/Mn<sup>4+</sup>), essential for antibacterial efficacy. SEM imaging displayed a unique flake-like morphology conducive to clustering, and EDS confirmed elemental composition. Notably, nitrogen sorption isotherms revealed a marked increase in surface area from 2 m²/g in pure HA to 11–16 m²/g in Mn/Ce oxide/HA, which may enhance bacterial interaction. Antibacterial assays demonstrated potent activity against <i>Bacillus cereus</i> (<i>B. cereus</i>), <i>Staphylococcus aureus (S. aureus)</i>, <i>Staphylococcus epidermidis (S. epidermidis)</i>, <i>Escherichia coli (E. coli)</i>, and <i>Salmonella typhi (S. typhi)</i>, linked to reactive oxygen species production and bacterial membrane disruption. This study highlights the robust structural and antibacterial features of Mn/Ce oxide/HA composites, advancing their suitability for biomedical applications, particularly in infection-resistant materials and bone grafts.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":618,"journal":{"name":"Journal of Cluster Science","volume":"36 1","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cluster Science","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s10876-024-02750-6","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}

引用次数: 0

Abstract

Mixed manganese/cerium oxide/hydroxyapatite composites are emerging as innovative materials with significant biomedical potential due to their antibacterial properties and biocompatibility. In this study, we synthesized mixed Mn/Ce oxide/HA composites using an ultrasonic-assisted sol-gel method, exploring their structural and functional characteristics through comprehensive analyses. Advanced characterization techniques, including X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), X-ray absorption spectroscopy (XAS), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and nitrogen adsorption-desorption isotherms, revealed a composite with enhanced structural stability and porosity, optimized for antibacterial applications. XRD confirmed the integration of a fluorite-structured CeO₂ phase with hexagonal hydroxyapatite, while FT-IR and XANES analyses verified the presence of functional phosphate groups and mixed oxidation states (Ce³⁺/Ce⁴⁺, Mn²⁺/Mn³⁺/Mn⁴⁺), essential for antibacterial efficacy. SEM imaging displayed a unique flake-like morphology conducive to clustering, and EDS confirmed elemental composition. Notably, nitrogen sorption isotherms revealed a marked increase in surface area from 2 m²/g in pure HA to 11–16 m²/g in Mn/Ce oxide/HA, which may enhance bacterial interaction. Antibacterial assays demonstrated potent activity against Bacillus cereus (B. cereus), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Escherichia coli (E. coli), and Salmonella typhi (S. typhi), linked to reactive oxygen species production and bacterial membrane disruption. This study highlights the robust structural and antibacterial features of Mn/Ce oxide/HA composites, advancing their suitability for biomedical applications, particularly in infection-resistant materials and bone grafts.

Graphical Abstract

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Cluster Science 化学-无机化学与核化学

CiteScore

6.70

自引率

0.00%

发文量

166

审稿时长

3 months

期刊介绍： The journal publishes the following types of papers: (a) original and important research; (b) authoritative comprehensive reviews or short overviews of topics of current interest; (c) brief but urgent communications on new significant research; and (d) commentaries intended to foster the exchange of innovative or provocative ideas, and to encourage dialogue, amongst researchers working in different cluster disciplines.