Hairpin aptamer and ROS-sensitive microcapsule-mediated glycoprotein determination for the prognosis of colorectal cancer

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-12-21 DOI:10.1007/s00604-024-06885-1

Xingjie Wu, Shasha Xiong, Ling Tao, Jian Huang, Xiangchun Shen

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Abstract

A novel glycoprotein assay was developed by integrating the hairpin aptamer (H-APT)–mediated glycoprotein recognition and the reactive oxygen species–sensitive microcapsule (ROS-MC)–induced signal amplification. The analyzing process begins with the transfer of the target glycoprotein to a chlorin e6 (Ce6)–labeled DNA sequence via H-APT-mediated DNA displacement. Subsequently, the Ce6-labeled DNA was used to induce the disassembly of fluorophore-loaded ROS-MC under 650-nm light irradiation. Leveraging the rapid release of the fluorophore and the high loading capacity of the MC, this glycoprotein assay is capable of quantifying glycoprotein content in native biofluids within 2.5 h, achieving a detection limit of 0.034 ng/mL. We applied this assay to determine the glycoprotein composition in plasma samples of colorectal cancer patients, revealing a significant increase in glycoprotein content for those with a poor prognosis. In summary, we have developed an innovative method for glycoprotein determination that shows potential for clinical translation.

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.