Deep profiling deconstructs features associated with memory CD8+ T cell tissue residence

IF 25.5 1区 医学 Q1 IMMUNOLOGY
Milcah C. Scott, Zoë Steier, Mark J. Pierson, J. Michael Stolley, Stephen D. O’Flanagan, Andrew G. Soerens, Sathi P. Wijeyesinghe, Lalit K. Beura, Gayathri Dileepan, Brandon J. Burbach, Marco Künzli, Clare F. Quarnstrom, Olivia C. Ghirardelli Smith, Eyob Weyu, Sara E. Hamilton, Vaiva Vezys, Alex K. Shalek, David Masopust
{"title":"Deep profiling deconstructs features associated with memory CD8+ T cell tissue residence","authors":"Milcah C. Scott, Zoë Steier, Mark J. Pierson, J. Michael Stolley, Stephen D. O’Flanagan, Andrew G. Soerens, Sathi P. Wijeyesinghe, Lalit K. Beura, Gayathri Dileepan, Brandon J. Burbach, Marco Künzli, Clare F. Quarnstrom, Olivia C. Ghirardelli Smith, Eyob Weyu, Sara E. Hamilton, Vaiva Vezys, Alex K. Shalek, David Masopust","doi":"10.1016/j.immuni.2024.11.007","DOIUrl":null,"url":null,"abstract":"Tissue-resident memory CD8<sup>+</sup> T (Trm) cells control infections and cancer and are defined by their lack of recirculation. Because migration is difficult to assess, residence is usually inferred by putative residence-defining phenotypic and gene signature proxies. We assessed the validity and universality of residence proxies by integrating mouse parabiosis, multi-organ sampling, intravascular staining, acute and chronic infection models, dirty mice, and single-cell multi-omics. We report that memory T cells integrate a constellation of inputs—location, stimulation history, antigen persistence, and environment—resulting in myriad differentiation states. Thus, current Trm-defining methodologies have implicit limitations, and a universal residence-specific signature may not exist. However, we define genes and phenotypes that more robustly correlate with tissue residence across the broad range of conditions that we tested. This study reveals broad adaptability of T cells to diverse stimulatory and environmental inputs and provides practical recommendations for evaluating Trm cells.","PeriodicalId":13269,"journal":{"name":"Immunity","volume":"22 1","pages":""},"PeriodicalIF":25.5000,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunity","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.immuni.2024.11.007","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Tissue-resident memory CD8+ T (Trm) cells control infections and cancer and are defined by their lack of recirculation. Because migration is difficult to assess, residence is usually inferred by putative residence-defining phenotypic and gene signature proxies. We assessed the validity and universality of residence proxies by integrating mouse parabiosis, multi-organ sampling, intravascular staining, acute and chronic infection models, dirty mice, and single-cell multi-omics. We report that memory T cells integrate a constellation of inputs—location, stimulation history, antigen persistence, and environment—resulting in myriad differentiation states. Thus, current Trm-defining methodologies have implicit limitations, and a universal residence-specific signature may not exist. However, we define genes and phenotypes that more robustly correlate with tissue residence across the broad range of conditions that we tested. This study reveals broad adaptability of T cells to diverse stimulatory and environmental inputs and provides practical recommendations for evaluating Trm cells.

Abstract Image

深度剖析解构了与记忆CD8+ T细胞组织驻留相关的特征
组织驻留记忆CD8+ T (Trm)细胞控制感染和癌症,并被定义为缺乏再循环。由于迁移难以评估,居住通常是通过假定的居住定义表型和基因标记代理来推断的。我们通过整合小鼠异种共生、多器官取样、血管内染色、急性和慢性感染模型、脏小鼠和单细胞多组学来评估驻留代理的有效性和普遍性。我们报道记忆T细胞整合了一系列输入-位置,刺激历史,抗原持久性和环境-导致无数的分化状态。因此,当前的trm定义方法具有隐含的局限性,并且可能不存在通用的特定于住所的签名。然而,我们定义了基因和表型,这些基因和表型与我们测试的广泛条件下的组织驻留更紧密相关。该研究揭示了T细胞对各种刺激和环境输入的广泛适应性,并为评估Trm细胞提供了实用建议。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Immunity
Immunity 医学-免疫学
CiteScore
49.40
自引率
2.20%
发文量
205
审稿时长
6 months
期刊介绍: Immunity is a publication that focuses on publishing significant advancements in research related to immunology. We encourage the submission of studies that offer groundbreaking immunological discoveries, whether at the molecular, cellular, or whole organism level. Topics of interest encompass a wide range, such as cancer, infectious diseases, neuroimmunology, autoimmune diseases, allergies, mucosal immunity, metabolic diseases, and homeostasis.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信