Intramuscular fatty infiltration and its correlation with muscle composition and function in hip osteoarthritis.

IF 5.3 2区医学 Q2 CELL BIOLOGY

Skeletal Muscle Pub Date : 2024-12-19 DOI:10.1186/s13395-024-00364-0

Tatiane Gorski, Nicola C Casartelli, Gillian Fitzgerald, Astrid M H Horstman, Evi Masschelein, Kalliopi J Essers, Nicola A Maffiuletti, Reto Sutter, Michael Leunig, Katrien De Bock

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引用次数: 0

Abstract

Background: Hip osteoarthritis patients display higher levels of fatty infiltration (FI) in the gluteus minimus (GM) compared to other hip muscles. We investigated specific histological factors such as fiber type composition and collagen deposition, and functional outcomes like muscle strength and activation associated with FI in these patients.

Methods: In twelve men (67 ± 6 y) undergoing total hip replacement (THR), hip and knee muscle strength and activation (electromyography, EMG) were assessed bilaterally. Magnetic resonance imaging (MRI) was used to compare the relative FI area and muscle cross sectional area (CSA) of the GM, rectus femoris (RF), tensor fascia latae (TFL) and vastus lateralis (VL). Adipocyte content, fiber type composition, grouping, fiber size, centrally nucleated fiber frequency, collagen deposition, satellite cell density and capillarization were assessed in intraoperative biopsies of the four muscles. Differences between GM and other muscles were assessed with repeated-measures one-way ANOVA followed by Dunnett's post-hoc test. Pearson coefficients were calculated for the correlations between FI measurements and the other histological and functional parameters.

Results: Strength was lower in the affected limb. Knee extensor weakness was accompanied by lower VL muscle activation. Muscle CSA and FI did not differ between sides. In the affected limb, GM displayed larger relative FI area (MRI) compared to RF and VL. Biopsy adipocyte content was higher in GM than RF and TFL. Compared to the other hip muscles, GM displayed higher type 1 fiber content while its type 2X fiber content was lower. Fiber grouping levels were higher in GM than the other muscles. Collagen content was higher in GM than TFL and VL. FI in GM was associated with type 1 (r = 0.43) and type 2X (r = -0.34) fiber content, fiber grouping (r = 0.39), and collagen deposition (r = 0.37). FI in VL was negatively associated with maximal knee extension strength (r = -0.65).

Conclusions: In patients undergoing THR, the higher FI levels of GM compared to other hip muscles were associated with fiber type composition and grouping, and with higher collagen deposition. Experimental studies exploring these associations could potentially uncover new targets for the treatment of intramuscular FI and related impairments in muscle function.

Trial registration: KEK number: 2016-01852, date of registration: 12-4-2017.

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髋关节骨关节炎患者肌内脂肪浸润及其与肌肉成分和功能的关系。

背景：与其他髋关节肌肉相比，髋关节骨关节炎患者臀小肌（GM）脂肪浸润（FI）水平更高。我们研究了这些患者与FI相关的特定组织学因素，如纤维类型组成和胶原沉积，以及肌肉力量和激活等功能结果。方法：对12例（67±6岁）接受全髋关节置换术（THR）的男性患者进行双侧髋关节和膝关节肌力和激活（肌电图，EMG）评估。采用磁共振成像（MRI）比较GM、股直肌（RF）、阔筋膜张肌（TFL）和股外侧肌（VL）的相对FI面积和肌肉横截面积（CSA）。术中对4块肌肉进行活检，评估脂肪细胞含量、纤维类型组成、纤维分组、纤维大小、中心有核纤维频率、胶原沉积、卫星细胞密度和毛细血管化。GM和其他肌肉之间的差异采用重复测量的单因素方差分析和Dunnett事后检验进行评估。计算了FI测量值与其他组织学和功能参数之间的相关性的Pearson系数。结果：患肢力量降低。膝伸肌无力伴下VL肌活化。两侧肌肉CSA和FI无差异。在患肢中，与RF和VL相比，GM显示出更大的相对FI面积（MRI）。活检脂肪细胞含量在GM高于RF和TFL。与其他臀部肌肉相比，GM的1型纤维含量较高，而2X型纤维含量较低。GM肌肉的纤维分组水平高于其他肌肉。GM的胶原蛋白含量高于TFL和VL。GM的FI与1型（r = 0.43）和2X型（r = -0.34）纤维含量、纤维分组（r = 0.39）和胶原沉积（r = 0.37）相关。VL的FI与最大膝关节伸展强度呈负相关（r = -0.65）。结论：在接受THR的患者中，与其他髋关节肌肉相比，GM较高的FI水平与纤维类型组成和分组以及较高的胶原沉积有关。探索这些关联的实验研究可能会发现治疗肌内FI和相关肌肉功能损伤的新靶点。试验报名：KEK号：2016-01852，报名日期：12-4-2017。

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来源期刊

Skeletal Muscle CELL BIOLOGY-

CiteScore

9.10

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： The only open access journal in its field, Skeletal Muscle publishes novel, cutting-edge research and technological advancements that investigate the molecular mechanisms underlying the biology of skeletal muscle. Reflecting the breadth of research in this area, the journal welcomes manuscripts about the development, metabolism, the regulation of mass and function, aging, degeneration, dystrophy and regeneration of skeletal muscle, with an emphasis on understanding adult skeletal muscle, its maintenance, and its interactions with non-muscle cell types and regulatory modulators. Main areas of interest include: -differentiation of skeletal muscle- atrophy and hypertrophy of skeletal muscle- aging of skeletal muscle- regeneration and degeneration of skeletal muscle- biology of satellite and satellite-like cells- dystrophic degeneration of skeletal muscle- energy and glucose homeostasis in skeletal muscle- non-dystrophic genetic diseases of skeletal muscle, such as Spinal Muscular Atrophy and myopathies- maintenance of neuromuscular junctions- roles of ryanodine receptors and calcium signaling in skeletal muscle- roles of nuclear receptors in skeletal muscle- roles of GPCRs and GPCR signaling in skeletal muscle- other relevant aspects of skeletal muscle biology. In addition, articles on translational clinical studies that address molecular and cellular mechanisms of skeletal muscle will be published. Case reports are also encouraged for submission. Skeletal Muscle reflects the breadth of research on skeletal muscle and bridges gaps between diverse areas of science for example cardiac cell biology and neurobiology, which share common features with respect to cell differentiation, excitatory membranes, cell-cell communication, and maintenance. Suitable articles are model and mechanism-driven, and apply statistical principles where appropriate; purely descriptive studies are of lesser interest.