Bioprospecting secondary metabolites with antimicrobial properties from soil bacteria in high-temperature ecosystems.

IF 4.3 2区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Sisay Demisie, Dong-Chan Oh, Adugna Abera, Geremew Tasew, Gizaw Dabessa Satessa, Fetene Fufa, Abebe Mekuria Shenkutie, Dawit Wolday, Ketema Tafess
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引用次数: 0

Abstract

Background: The ongoing emergence and spread of drug-resistant pathogens necessitate urgent solutions. Natural products from bacterial sources are recognized as a promising source of antibiotics. This study aimed to isolate and characterize soil microorganisms from extremely hot environments and to screen their secondary metabolites for antibacterial activity.

Methods: Bacterial isolates were identified using standard culture techniques. Primary and secondary screenings for antimicrobial activity were conducted using the Modified Kirby-Bauer antibiotic susceptibility test against five bacterial species. Based on the efficacy of antimicrobial activity against these target pathogens, the isolate Pseudomonas sp. strain ASTU00105 was selected for further characterization through whole genomic sequencing. Secondary metabolites were analyzed using GC-MS, and antioxidant activities were also evaluated.

Results: A total of 76 isolates were identified, and their secondary metabolites were tested against Escherichia coli, Salmonella typhi, Acinetobacter baumannii, Staphylococcus aureus, Streptococcus pyogenes, and Candida albicans. Seventeen isolates (22.37%) exhibited antimicrobial activity. Isolate ASTU00105 exhibited the highest activity against all the test organisms and was selected for further analysis. Whole-genome sequencing using the Nanopore MinION sequencer revealed that strain ASTU00105 belonged to the genus Pseudomonas with the highest similarity (95.97%) to Pseudomonas stutzeri, and designated as Pseudomonas sp. strain ASTU00105. Upon Average Nucleotide Identity (ANI) analysis, the strain exhibited 87.81% sequence similarity with genes of the closest type strain, suggesting its novelty and distinctiveness within the Pseudomonas genus. The genomic analysis of the isolated strain revealed 6 biosynthetic gene cluster (BGC) genes dispersed throughout the entire genome, which are implicated in the synthesis of antimicrobial secondary metabolites. The major chemical compounds detected in the EtAc extracts as detected by gas chromatography-mass spectrometry (GC-MS) were phenol, 2,5-bis (1,1-dimethylethyl) (36.6%), followed by 1,2-Benzenedicarboxylic acid, diethyl ester (12.22%), Eicosane (9.71%), Dibutyl phthalate (3.93%), and 1-Dodecanol (2.34%).

In conclusion: Pseudomonas sp. strain ASTU00105 exhibited the greatest potential for producing secondary metabolites with significant antimicrobial activity.

高温生态系统土壤细菌抗菌次生代谢物的生物勘探。
背景:耐药病原体的不断出现和传播需要紧急解决办法。细菌来源的天然产物被认为是有前途的抗生素来源。本研究旨在从极热环境中分离和表征土壤微生物,并筛选其次级代谢产物的抗菌活性。方法:采用标准培养技术对分离的细菌进行鉴定。采用改良Kirby-Bauer药敏试验对5种细菌进行了一次和二次抗菌活性筛选。基于对这些目标病原体的抗菌活性,选择假单胞菌菌株ASTU00105进行全基因组测序进一步鉴定。用GC-MS分析其次生代谢产物,并评价其抗氧化活性。结果:共鉴定出76株分离株,并检测了其对大肠杆菌、伤寒沙门氏菌、鲍曼不动杆菌、金黄色葡萄球菌、化脓性链球菌和白色念珠菌的次生代谢产物。17株(22.37%)具有抗菌活性。分离物ASTU00105对所有试验生物的活性最高,并被选中进行进一步分析。利用纳米孔MinION测序仪对菌株ASTU00105进行全基因组测序,结果显示菌株ASTU00105属于假单胞菌属,与stutzeri假单胞菌的相似性最高(95.97%),并将其命名为Pseudomonas sp.菌株ASTU00105。经平均核苷酸同一性(ANI)分析,该菌株与最近型菌株的基因序列相似性为87.81%,表明其在假单胞菌属中具有新颖性和独特性。对分离菌株的基因组分析发现,6个生物合成基因簇(BGC)基因分布在整个基因组中,这些基因与抗菌次级代谢物的合成有关。气相色谱-质谱联用(GC-MS)法检测到的主要化合物为苯酚、2,5-二(1,1-二甲基乙基)(36.6%),其次为1,2-苯二甲酸二乙酯(12.22%)、二烷(9.71%)、邻苯二甲酸二丁酯(3.93%)和1-十二醇(2.34%)。综上所述,ASTU00105假单胞菌具有较强的次生代谢产物活性。
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来源期刊
Microbial Cell Factories
Microbial Cell Factories 工程技术-生物工程与应用微生物
CiteScore
9.30
自引率
4.70%
发文量
235
审稿时长
2.3 months
期刊介绍: Microbial Cell Factories is an open access peer-reviewed journal that covers any topic related to the development, use and investigation of microbial cells as producers of recombinant proteins and natural products, or as catalyzers of biological transformations of industrial interest. Microbial Cell Factories is the world leading, primary research journal fully focusing on Applied Microbiology. The journal is divided into the following editorial sections: -Metabolic engineering -Synthetic biology -Whole-cell biocatalysis -Microbial regulations -Recombinant protein production/bioprocessing -Production of natural compounds -Systems biology of cell factories -Microbial production processes -Cell-free systems
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