Expression of Major Basic Protein and Endothelial Adhesion Molecules in Chronically Inflamed Mucosa of the Ethmoid Labyrinth

IF 3.1 4区医学 Q3 IMMUNOLOGY

Immunity, Inflammation and Disease Pub Date : 2024-12-19 DOI:10.1002/iid3.70066

Tijana Vukadinović, Biserka Vukomanović Đurđević, Aleksandar Perić

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引用次数: 0

Abstract

Background/Objectives

Tissue remodeling, including dense eosinophil infiltration, is essential for forming inflammatory nasal polyps (NPs) and the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). Toxic eosinophil major basic protein (MBP) damages the sinus mucosa epithelium and lamina propria, which initiates reparative processes leading to tissue remodeling. MBP specifically binds to BMK-13 antibodies allowing immunohistochemical (IHC) tissue staining for eosinophils. This study evaluated the association between NP stromal BMK-13 and endothelial adhesion molecule staining, and clinical parameters of NP patients compared to IHC expression and clinical parameters in subjects with healthy nasal mucosa.

Methods

We included 30 patients with bilateral NPs who were selected for endoscopic ethmoidectomy. The control group was of 30 subjects with non-inflamed nasal mucosa but with middle turbinate aeration, chosen for surgery. All participants were clinically scored before surgery, according to quality of life (QoL) outcome and symptoms. The degree of disease extension on computed tomography scans of the paranasal sinuses was also evaluated. Tissue samples after surgery were IHC stained for BMK-13 and endothelial proliferation markers CD31 and CD34.

Results

Expression of BMK-13, CD31, and CD34 in tissues of NPs was higher than in healthy nasal mucosa. Positive correlations were observed between BMK-13 expression, impaired QoL, and radiologically assessed extension of inflammation in NP patients.

Conclusion

Apart from the fact that the NP tissue has, as expected, more intense eosinophilic infiltration, the proliferation of blood vessels is more pronounced in the NP tissue than in the tissue of healthy nasal mucosa. Expression of MBP in the tissue of ethmoidal NPs could serve as a potential marker of the degree of expansion of CRSwNP and indicate the severity of the disease.

Clinical Trial Registration

None, because it was a cross-sectional study.

Abstract Image

查看原文本刊更多论文

筛迷宫慢性炎症黏膜中主要碱性蛋白和内皮粘附分子的表达。

背景/目的：组织重塑，包括密集的嗜酸性粒细胞浸润，是炎症性鼻息肉（NPs）形成和慢性鼻窦炎伴鼻息肉（CRSwNP）发病的必要条件。毒性嗜酸性粒细胞大碱性蛋白（MBP）损害窦黏膜上皮和固有层，启动修复过程导致组织重塑。MBP特异性结合BMK-13抗体，允许免疫组化（IHC）组织染色嗜酸性粒细胞。本研究比较了NP间质BMK-13与内皮粘附分子染色、NP患者临床参数与健康鼻黏膜IHC表达和临床参数的关系。方法：我们选择30例双侧NPs患者行内镜下筛切除术。对照组为30例鼻黏膜无炎症但经中鼻甲通气的患者，选择手术治疗。所有参与者在手术前根据生活质量（QoL）结果和症状进行临床评分。我们还评估了鼻窦计算机断层扫描的疾病扩展程度。术后组织标本进行免疫组化染色，检测BMK-13和内皮细胞增殖标志物CD31和CD34。结果：NPs组织中BMK-13、CD31和CD34的表达高于正常鼻黏膜组织。在NP患者中，BMK-13表达、生活质量受损和放射学评估的炎症扩展之间存在正相关。结论：除了NP组织如预期的那样有更强烈的嗜酸性粒细胞浸润外，NP组织中血管的增殖比健康鼻黏膜组织更明显。筛状NPs组织中MBP的表达可作为CRSwNP扩增程度的潜在标志，提示疾病的严重程度。临床试验注册：没有，因为这是一项横断面研究。

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来源期刊

Immunity, Inflammation and Disease Medicine-Immunology and Allergy

CiteScore

3.60

自引率

0.00%

发文量

146

审稿时长

8 weeks

期刊介绍： Immunity, Inflammation and Disease is a peer-reviewed, open access, interdisciplinary journal providing rapid publication of research across the broad field of immunology. Immunity, Inflammation and Disease gives rapid consideration to papers in all areas of clinical and basic research. The journal is indexed in Medline and the Science Citation Index Expanded (part of Web of Science), among others. It welcomes original work that enhances the understanding of immunology in areas including: • cellular and molecular immunology • clinical immunology • allergy • immunochemistry • immunogenetics • immune signalling • immune development • imaging • mathematical modelling • autoimmunity • transplantation immunology • cancer immunology