Evaluation of the effect of melatonin treatment on telomere length of the retinal pigment epithelium in streptozotocin-induced diabetic rat model.

IF 1.7 4区医学 Q3 OPHTHALMOLOGY

BMC Ophthalmology Pub Date : 2024-12-18 DOI:10.1186/s12886-024-03732-y

Ayla Eren Ozdemir

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Abstract

Objectives: We aimed to investigate the effect of diabetic retinopathy and melatonin treatment on the relative telomer lengths (RTL) in retinal pigment epithelium (RPE) cells in a streptozotocin-induced diabetic rat model.

Background: TL can be used to evaluate diabetes mellitus, its complications, and the effectiveness of its treatment. However, TL assessment has not been performed in retinal cells in a diabetic retinopathy model until now.

Methods: Forty Sprague-Dawley male rats were randomly divided into four groups. The experimental groups were: Control Group (C): non- diabetic rats; Diabetes Mellitus Group (DM): rats induced to diabetes without treatment; Melatonin and Diabetes Mellitus Group (Mel + DM): rats induced to diabetes and after confirmation, treated with melatonin; Melatonin Group (Mel): rats were not induced to diabetes, treated with melatonin. Diabetes was induced by intraperitoneal administration of streptozotocin solution after 12 h food fasting. For eight weeks after the diabetes was induced, melatonin was administered via subcutaneous injection at a dose of 10 mg / kg. RTLs were measured by qPCR method with modifications. The comparison of averaged data among groups was performed using least significant difference (LSD) and Kruskal - Wallis Test and One way ANOVA test.

Results: RTL was significantly similar in control and melatonin group. RTL was thinnest in DM group, in addition melatonin treatment significantly prevented the RTL shortening in DM + Mel group (p = 0.031).

Conclusion: We demonstrated that diabetic retinopathy led to the shortening of RTL in RPE cells in rats and melatonin treatment prevents this shortening.

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褪黑素对链脲佐菌素诱导的糖尿病大鼠视网膜色素上皮端粒长度的影响。

目的：研究糖尿病视网膜病变和褪黑素治疗对链脲佐菌素诱导的糖尿病大鼠视网膜色素上皮（RPE）细胞相对端粒长度（RTL）的影响。背景：TL可用于评价糖尿病及其并发症及治疗效果。然而，到目前为止，尚未对糖尿病视网膜病变模型的视网膜细胞进行TL评估。方法：40只雄性Sprague-Dawley大鼠随机分为4组。实验组分别为：对照组(C)：非糖尿病大鼠；糖尿病组（DM）：未经治疗诱导的糖尿病大鼠；褪黑素与糖尿病组（Mel + DM）：糖尿病诱导大鼠，确认后给予褪黑素治疗；褪黑素组（Mel）：未诱导大鼠糖尿病，给予褪黑素治疗。禁食12 h后腹腔注射链脲佐菌素诱导糖尿病。诱导糖尿病8周后，皮下注射褪黑激素，剂量为10mg / kg。采用修饰后的qPCR法测定rtl。各组平均资料比较采用最小显著差异（LSD）、Kruskal - Wallis检验和单因素方差分析（One - way ANOVA）检验。结果：对照组与褪黑素组RTL差异有统计学意义。DM组RTL最薄，褪黑素治疗显著阻止DM + Mel组RTL缩短（p = 0.031）。结论：我们证明糖尿病视网膜病变导致大鼠RPE细胞RTL缩短，褪黑素治疗可防止这种缩短。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Ophthalmology OPHTHALMOLOGY-

CiteScore

3.40

自引率

5.00%

发文量

441

审稿时长

6-12 weeks

期刊介绍： BMC Ophthalmology is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of eye disorders, as well as related molecular genetics, pathophysiology, and epidemiology.