Distinct contributions of BDNF/MEK/ERK1/2 signaling pathway components to whisker-dependent tactile learning and memory.

Abstract

Whisker-mediated tactile perception is essential for rodent navigation, food acquisition, and social interactions. However, the molecular mechanisms underlying tactile information processing, learning, and memory have not been studied to the same extent as for other modalities. Using immunohistochemical staining, we investigated changes in regional c-Fos expression as an index of neuronal activity and phosphorylated (p)ERK1/2 as an index of ERK1/2 activity in mice trained on a tactile-cued 8-arm radial maze task. Over 12 trials, mice learned to selectively explore four baited arms covered with wire as the tactile cue while avoiding un-baited uncovered arms. The density of c-Fos⁺ cells was significantly higher in somatosensory cortex but not frontal cortex or amygdala of mice exposed to tactile cue - bait pairing compared to mice exposed to the same maze with all arms baited with or without tactile cues (unpaired conditions). The density of pERK1/2⁺ cells was also increased after paired trials 7 and 12 but not after paired trials 1 and 3 in frontal cortex, amygdala, and somatosensory cortex compared to mice exposed to the unpaired condition. The MEK1/2 inhibitor SL327 reduced c-Fos expression in frontal cortex and amygdala when applied during early trials, but impaired working memory when applied before later trials without affecting c-Fos expression. Heterozygous BDNF knockout mice exhibited impaired task learning and reduced pERK1/2 expression in frontal cortex and amygdala but not somatosensory cortex. These findings suggest that the BDNF/MEK/ERK1/2 pathway selectively promotes memory trace formation in frontal cortex and amygdala but not encoding in somatosensory cortex.