Angela M Moro, Natália Brucker, Gabriela Goethel, Ingrid Flesch, Sabrina Nascimento, Mariele Charão, Bruna Gauer, Elisa Sauer, Larissa V Cestonaro, Gabriel Pedroso Viçozzi, Adriana Gioda, Tatiana D Saint'Pierre, Marcelo D Arbo, Ingrid Garcia, Shanda A Cattani, Rodrigo R Petrecelli, Mirkos Ortiz Martins, Solange Cristina Garcia
{"title":"The Influence of Blood Titanium Levels on DNA Damage in Brazilian Workers Occupationally Exposed to Different Chemical Agents.","authors":"Angela M Moro, Natália Brucker, Gabriela Goethel, Ingrid Flesch, Sabrina Nascimento, Mariele Charão, Bruna Gauer, Elisa Sauer, Larissa V Cestonaro, Gabriel Pedroso Viçozzi, Adriana Gioda, Tatiana D Saint'Pierre, Marcelo D Arbo, Ingrid Garcia, Shanda A Cattani, Rodrigo R Petrecelli, Mirkos Ortiz Martins, Solange Cristina Garcia","doi":"10.1007/s12011-024-04472-2","DOIUrl":null,"url":null,"abstract":"<p><p>Occupational exposure to pollutants may cause health-damaging effects in humans. Genotoxicity assays can be used to detect the toxic effects of pollutants. In the present study, we evaluated genetic damage in three populations occupationally exposed to benzene, pyrenes, and agrochemicals and assessed the possible influence of titanium (Ti) co-exposure. A total of 275 subjects were enrolled in this study. The occupationally exposed population was composed of 201 male individuals, divided into three different groups: gas station attendants (GSA group) (n = 76), taxi drivers (TD group) (n = 97), farmers (farmers group) (n = 28), and control (n = 74). Biomarkers of exposure and effect were investigated such as AChe, BuChE, t,t-muconic acid (t,t-MA), and 1-hydroxypyrene (1-OHP). Ti levels in blood were higher in all the workers compared with the control group. DNA damage evaluated by comet assay was higher in the taxi drivers and farmers than in the controls, and the frequency of micronucleate buccal cells was higher in the gas station attendants and taxi drivers than in the controls. Correlations were found among occupational exposure time and biomarkers of exposure, genotoxicity biomarkers, and blood Ti levels. Our results demonstrated Ti co-exposure in the gas station attendants, taxi drivers, and farmers, and blood Ti levels were linked with the respective biomarkers of exposure. Additionally, tools through machine learning corroborated these findings, and Ti was the factor that contributed to DNA damage. Thus, the present study indicates the role of Ti in occupational settings and interactions with already known major xenobiotics present in the occupational environment contributing to genotoxicity.</p>","PeriodicalId":8917,"journal":{"name":"Biological Trace Element Research","volume":" ","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Trace Element Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12011-024-04472-2","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Occupational exposure to pollutants may cause health-damaging effects in humans. Genotoxicity assays can be used to detect the toxic effects of pollutants. In the present study, we evaluated genetic damage in three populations occupationally exposed to benzene, pyrenes, and agrochemicals and assessed the possible influence of titanium (Ti) co-exposure. A total of 275 subjects were enrolled in this study. The occupationally exposed population was composed of 201 male individuals, divided into three different groups: gas station attendants (GSA group) (n = 76), taxi drivers (TD group) (n = 97), farmers (farmers group) (n = 28), and control (n = 74). Biomarkers of exposure and effect were investigated such as AChe, BuChE, t,t-muconic acid (t,t-MA), and 1-hydroxypyrene (1-OHP). Ti levels in blood were higher in all the workers compared with the control group. DNA damage evaluated by comet assay was higher in the taxi drivers and farmers than in the controls, and the frequency of micronucleate buccal cells was higher in the gas station attendants and taxi drivers than in the controls. Correlations were found among occupational exposure time and biomarkers of exposure, genotoxicity biomarkers, and blood Ti levels. Our results demonstrated Ti co-exposure in the gas station attendants, taxi drivers, and farmers, and blood Ti levels were linked with the respective biomarkers of exposure. Additionally, tools through machine learning corroborated these findings, and Ti was the factor that contributed to DNA damage. Thus, the present study indicates the role of Ti in occupational settings and interactions with already known major xenobiotics present in the occupational environment contributing to genotoxicity.
期刊介绍：
Biological Trace Element Research provides a much-needed central forum for the emergent, interdisciplinary field of research on the biological, environmental, and biomedical roles of trace elements. Rather than confine itself to biochemistry, the journal emphasizes the integrative aspects of trace metal research in all appropriate fields, publishing human and animal nutritional studies devoted to the fundamental chemistry and biochemistry at issue as well as to the elucidation of the relevant aspects of preventive medicine, epidemiology, clinical chemistry, agriculture, endocrinology, animal science, pharmacology, microbiology, toxicology, virology, marine biology, sensory physiology, developmental biology, and related fields.
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