Efficient extraction and analysis method for lead-containing nanoparticles in complex biological samples to eliminate "false" interferences by using SP-ICP-MS.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2024-12-17 DOI:10.1016/j.talanta.2024.127372

Qinfei Zhou, Lihong Liu, Junhui Zhang, Haozhong Tian, Hua Guo, Zhenhua Wang, Yong Liang, Bin He, Ligang Hu, Guibin Jiang

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引用次数: 0

Abstract

Metal-containing nanoparticles (MNPs) ubiquitously exist in the environment and organisms, playing distinct roles in the fate and toxicity of metals. However, the extraction and analysis of the MNPs in biological samples is still a great challenge and the interferences of other metal species and complex matrices remains unclear. In this work, we established a method for efficient extraction and accurate analysis of MNPs in biological samples to eliminate the interference caused by metal ions and biological matrices based on the alkali extraction and single particle mode inductively coupled plasma mass spectrometry (SP-ICP-MS). Obvious interference signals of lead-containing nanoparticles (PbNPs) were found in various biological matrices (liver, brain, bile, intestine, stomach), causing false positive results or overestimation of PbNPs. Then, a novel strategy using EDTA and ultrasonic during the TMAH extraction process were proposed to successfully eliminate the interferences due to the strong and competitively binding of EDTA to Pb ions, which was identified as ionic signals in SP-ICP-MS and resulted in the elimination of interferences. Finally, this method was successfully applied for the extraction, characterization and quantification of PbNPs in different biological tissues collected near a power plant, revealing the occurrence of PbNPs in stomach, intestine and liver tissues and indicating their oral exposure and potential translocation. This method could be universally applied for the efficient extraction and accurate analysis of MNPs in biological samples and thus provided a reliable and powerful tool for the investigation of the occurrence, fate and toxicity of MNPs in environmental and organisms.

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.