Investigation of bioprintable modified agar-based hydrogels with antimicrobial properties

IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Edyta Piłat , Przemysław Gnatowski , Agnieszka Kurdyn , Hubert Cieśliński , Ewa Augustin , Justyna Kucińska-Lipka
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Abstract

Due to the numerous dangers arising from excessive use of antibiotics in treatments, researchers have been searching for natural alternatives to conventional antibiotics. Despite the popularity of plant extracts, essential oils, and their derivatives in herbal medicine, their applications in novel therapies are rather limited. This paper tries to open a new possibility for infection treatments by assessing the suitability of antimicrobial hydrogels as bioinks. Antimicrobial activity against S. epidermidis, P. aeruginosa, S. aureus, E. coli of selected extracts and geraniol were investigated. Suitable agent was incorporated into agar-based hydrogel. Physicochemical properties of the obtained compositions were analyzed, including determination of swelling kinetics and key polymer network parameters, contact angle measurements, FTIR spectra analysis, biocompatibility assessment, antimicrobial tests and bioprintability studies. Results confirmed geraniol's superior antimicrobial activity in pure form and in hydrogels. The obtained materials showed high swelling capacity, satisfying extrusion processability, shape fidelity, and great biocompatibility in their unmodified state. Nevertheless, modification with geraniol caused a significant decrease of cell viability, which limits their usage as bioinks in current form, due to the cytotoxic effect on cells. To improve cells interactions, studies on materials with geraniol and other agents with similar mechanism should be conducted in the future.

Abstract Image

生物可打印改性琼脂基抗菌水凝胶的研究。
由于在治疗中过度使用抗生素会产生许多危险,研究人员一直在寻找传统抗生素的天然替代品。尽管植物提取物、精油及其衍生物在草药中很受欢迎,但它们在新疗法中的应用却相当有限。本文试图通过评价抗菌水凝胶作为生物墨水的适用性,为感染治疗开辟一种新的可能性。研究了所选提取物和香叶醇对表皮葡萄球菌、铜绿假单胞菌、金黄色葡萄球菌、大肠杆菌的抑菌活性。在琼脂基水凝胶中加入合适的助剂。对所得组合物的理化性质进行了分析,包括溶胀动力学和关键聚合物网络参数的测定、接触角测量、FTIR光谱分析、生物相容性评估、抗菌试验和生物可打印性研究。结果证实了香叶醇在纯形式和水凝胶中具有较好的抗菌活性。所得材料在未修饰状态下具有较高的膨胀能力、良好的挤压加工性、形状保真度和良好的生物相容性。然而,由于对细胞的细胞毒性作用,用香叶醇修饰导致细胞活力显著降低,这限制了它们作为当前形式的生物墨水的使用。为了改善细胞间的相互作用,未来还需要对材料与香叶醇等具有类似机制的药物进行研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Biological Macromolecules
International Journal of Biological Macromolecules 生物-生化与分子生物学
CiteScore
13.70
自引率
9.80%
发文量
2728
审稿时长
64 days
期刊介绍: The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.
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